Solid phase synthesis of 5′-diphosphorylated oligoribonucleotides and their conversion to capped m7 Gppp-oligoribonucleotides for U.S. as primers for influenza A virus RNA polymerase in vitro

G. G. Brownlee; E. Fodor; D. C. Pritlove; K. G. Gould; J. J. Dalluge

doi:10.1093/nar/23.14.2641

Solid phase synthesis of 5′-diphosphorylated oligoribonucleotides and their conversion to capped m⁷ Gppp-oligoribonucleotides for U.S. as primers for influenza A virus RNA polymerase in vitro

G. G. Brownlee, E. Fodor, D. C. Pritlove, K. G. Gould, J. J. Dalluge

Chemistry (Twin Cities)

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

We have synthesized four different 5′-diphosphorylated oligoribonucleotides, varying in length from 11 to 13 nucleotides by a new solid phase method. After deprotectlon and partial purification the 5′-diphosphorylated oligoribonucleotides could be converted to capped (m⁷Gppp)-oligoribonucleotides using guanylyl transferase. Radlolabelled capped oligoribonucleotides acted as primers for the influenza A virus RNA polymerase in vitro. The solid phase method described here should also allow the addition of 5′-diphosphates to synthetic ollgodeoxyribonucleotides and be capable of automation.

Original language	English (US)
Pages (from-to)	2641-2647
Number of pages	7
Journal	Nucleic acids research
Volume	23
Issue number	14
DOIs	https://doi.org/10.1093/nar/23.14.2641
State	Published - Jul 25 1995

Bibliographical note

Funding Information:
We thank Prof. G. Lowe for originally suggesting the phosphorylation method and for advice and Dr T. Claridge for the3IP NMR analysis. We acknowledge the encouragement of Drs M. Gait and B. Sproat This work was supported by an MRC project grant to GGB and an NIH grant GM29812 to Prof. J. A. McCloskey (University of Utah).

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10.1093/nar/23.14.2641

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Solid phase synthesis of 5′-diphosphorylated oligoribonucleotides and their conversion to capped m⁷ Gppp-oligoribonucleotides for U.S. as primers for influenza A virus RNA polymerase in vitro. / Brownlee, G. G.; Fodor, E.; Pritlove, D. C. et al.
In: Nucleic acids research, Vol. 23, No. 14, 25.07.1995, p. 2641-2647.

Research output: Contribution to journal › Article › peer-review

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abstract = "We have synthesized four different 5′-diphosphorylated oligoribonucleotides, varying in length from 11 to 13 nucleotides by a new solid phase method. After deprotectlon and partial purification the 5′-diphosphorylated oligoribonucleotides could be converted to capped (m7Gppp)-oligoribonucleotides using guanylyl transferase. Radlolabelled capped oligoribonucleotides acted as primers for the influenza A virus RNA polymerase in vitro. The solid phase method described here should also allow the addition of 5′-diphosphates to synthetic ollgodeoxyribonucleotides and be capable of automation.",

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