Solid-Phase Assembly of Multienzyme Systems into Artificial Cellulosomes

Nicoll Zeballos, Eleftheria Diamanti, Ana I. Benítez-Mateos, Claudia Schmidt-Dannert, Fernando López-Gallego

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


We herein describe a bioinspired solid-phase assembly of a multienzyme system scaffolded on an artificial cellulosome. An alcohol dehydrogenase and an ω-transaminase were fused to cohesin and dockerin domains to drive their sequential and ordered coimmobilization on agarose porous microbeads. The resulting immobilized scaffolded enzymatic cellulosome was characterized through quartz crystal microbalance with dissipation and confocal laser scanning microscopy to demonstrate that both enzymes interact with each other and physically colocalize within the microbeads. Finally, the assembled multifunctional heterogeneous biocatalyst was tested for the one-pot conversion of alcohols into amines. By using the physically colocalized enzymatic system confined into porous microbeads, the yield of the corresponding amine was 1.3 and 10 times higher than the spatially segregated immobilized system and the free enzymes, respectively. This work establishes the basis of a new concept to organize multienzyme systems at the nanoscale within solid and porous immobilization carriers.

Original languageEnglish (US)
Pages (from-to)1966-1972
Number of pages7
JournalBioconjugate Chemistry
Issue number9
StatePublished - Sep 15 2021

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© 2021 American Chemical Society.


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