Solid-phase assays for the detection of alloantibody against human leukocyte antigens: panacea or Pandora?

T Roberts, G Tumer, H M Gebel, R A Bray

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Serological assessments of antibodies directed against human leucocyte antigens (HLA) formed the basis of early histocompatibility testing (Patel & Terasaki, 1969 N Engl J Med, 280, 735). However, over the past decade, significant advances in HLA antibody detection technologies have emerged. The development and implementation of solid-phase assays has led to safer and more efficient allocation of organs by effectively distinguishing HLA from non-HLA antibodies. Although solid-phase assays are not standardized, they are widely accepted as the new 'gold standard'. However, this technology is not without its challenges. This review is intended to provide a better understanding of solid-phase HLA antibody testing and will focus on important caveats associated with this evolving technology. Examples of the limitations of the technology as well as common data misinterpretations will be shown. Both of which could pose potential harm to transplant recipients (Tait et al., Transplantation, 95, 19).

Original languageEnglish (US)
Pages (from-to)362-9
Number of pages8
JournalInternational Journal of Immunogenetics
Issue number5
StatePublished - Oct 2014

Bibliographical note

© 2014 John Wiley & Sons Ltd.


  • Artifacts
  • HLA Antigens/immunology
  • Histocompatibility Testing
  • Humans
  • Immunoassay/methods
  • Isoantibodies/immunology
  • Reproducibility of Results

PubMed: MeSH publication types

  • Journal Article
  • Review


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