Slow Receptor Binding of the Noncytopathic HIV-2UC1 Envs Is Balanced by Long-Lived Activation State and Efficient Fusion Activity

Miranda Harris; Sneha Ratnapriya; Angela Chov; Héctor Cervera; Alisha Block; Christopher Gu; Nathaniel Talledge; Louis M. Mansky; Joseph Sodroski; Alon Herschhorn

doi:10.1016/j.celrep.2020.107749

Slow Receptor Binding of the Noncytopathic HIV-2_UC1 Envs Is Balanced by Long-Lived Activation State and Efficient Fusion Activity

Miranda Harris, Sneha Ratnapriya, Angela Chov, Héctor Cervera, Alisha Block, Christopher Gu, Nathaniel Talledge, Louis M. Mansky, Joseph Sodroski, Alon Herschhorn

Research output: Contribution to journal › Article › peer-review

Abstract

Harris et al. show that the envelope glycoproteins of a rare HIV-2_UC1 strain evolved to balance low affinity to the CD4 receptor with an improved, long-lived, and readily triggerable molecular machinery to mediate virus entry.

Original language	English (US)
Article number	107749
Journal	Cell reports
Volume	31
Issue number	10
DOIs	https://doi.org/10.1016/j.celrep.2020.107749
State	Published - Jun 9 2020

Bibliographical note

Funding Information:
We thank Eva Perez-Greene for helping to prepare the revised manuscript, as well as Paula Cannon and Nick Llewellyn for providing the HIV-2 ROD10 and HIV-2 ROD14 Env-expressing plasmids. A.H. is the recipient of an amfAR Mathilde Krim Fellowship in Basic Biomedical Research ( 108501-53-RKNT ) and a phase II amfAR research grant ( 109285-58-RKVA ) for independent investigators. This work was supported by internal funds of the Department of Medicine at the University of Minnesota and by NIH/NIDA grant 1DP2DA049279-01 (to A.H.). N.T. was supported by NIH F32 DA007097 , and L.M.M. was supported by NIH R01 AI150468 . Molecular graphics and analyses were performed with UCSF Chimera, which is supported by NIH P41-GM103311 . The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH.

Keywords

HIV envelope glycoproteins
binding kinetics
long-lived activation state
molecular mechanism of HIV entry

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

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Slow Receptor Binding of the Noncytopathic HIV-2_UC1 Envs Is Balanced by Long-Lived Activation State and Efficient Fusion Activity. / Harris, Miranda; Ratnapriya, Sneha; Chov, Angela; Cervera, Héctor; Block, Alisha; Gu, Christopher; Talledge, Nathaniel; Mansky, Louis M.; Sodroski, Joseph; Herschhorn, Alon.

In: Cell reports, Vol. 31, No. 10, 107749, 09.06.2020.

Research output: Contribution to journal › Article › peer-review

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abstract = "Harris et al. show that the envelope glycoproteins of a rare HIV-2UC1 strain evolved to balance low affinity to the CD4 receptor with an improved, long-lived, and readily triggerable molecular machinery to mediate virus entry.",

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