Slow glycinergic transmission mediated by transmitter pooling

Veeramuthu Balakrishnan, Sidney P. Kuo, Patrick D. Roberts, Laurence O. Trussell

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Most fast-acting neurotransmitters are rapidly cleared from synaptic regions. This feature isolates synaptic sites, rendering the time course of synaptic responses independent of the number of active synapses. We found an exception at glycinergic synapses on granule cells of the rat dorsal cochlear nucleus. Here the duration of inhibitory postsynaptic currents (IPSCs) was dependent on the number of presynaptic axons that were stimulated and on the number of vesicles that were released from each axon. Increasing the stimulus number or frequency, or blocking glycine uptake, slowed synaptic decays, whereas a low-affinity competitive antagonist of glycine receptors (GlyRs) accelerated IPSC decay. These effects could be explained by unique features of GlyRs that are activated by pooling of glycine across synapses. Functionally, increasing the number of IPSPs markedly lengthened the period of spike inhibition following the cessation of presynaptic stimulation. Thus, temporal properties of inhibition can be controlled by activity levels in multiple presynaptic cells or by adjusting release probability at individual synapses.

Original languageEnglish (US)
Pages (from-to)286-294
Number of pages9
JournalNature neuroscience
Issue number3
StatePublished - Mar 2009
Externally publishedYes

Bibliographical note

Funding Information:
We thank H. Huang, C.E. Jahr and M.E. Roberts for comments on the manuscript and H. Sundaram for the gift of ORG-25543. This work was supported by US National Institutes of Health grant NS028901.


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