Slow and steady wins the race: physical limits on the rate of viral DNA packaging

Research output: Contribution to journalReview article

Abstract

During the assembly of dsDNA viruses such as the tailed bacteriophages and herpesviruses, the viral chromosome is compacted to near crystalline density inside a preformed head shell. DNA translocation is driven by powerful ring ATPase motors that couple ATP binding, hydrolysis, and release to force generation and movement. Studies of the motor of the bacteriophage phi29 have revealed a complex mechanochemistry behind this process that slows as the head fills. Recent studies of the physical behavior of packaging DNA suggest that surprisingly long-time scales of relaxation of DNA inside the head and jamming phenomena during packaging create the physical need for regulation of the rate of packaging. Studies of DNA packaging in viral systems have, therefore, revealed fundamental insight into the complex behavior of DNA and the need for biological systems to accommodate these physical constraints.

Original languageEnglish (US)
Pages (from-to)32-37
Number of pages6
JournalCurrent Opinion in Virology
Volume36
DOIs
StatePublished - Jun 2019

Fingerprint

DNA Packaging
Virus Assembly
Viral DNA
Head
Product Packaging
Bacteriophages
DNA
Herpesviridae
Adenosine Triphosphatases
Hydrolysis
Chromosomes
Adenosine Triphosphate

PubMed: MeSH publication types

  • Journal Article
  • Review
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

Slow and steady wins the race : physical limits on the rate of viral DNA packaging. / Jardine, Paul J.

In: Current Opinion in Virology, Vol. 36, 06.2019, p. 32-37.

Research output: Contribution to journalReview article

@article{83f533cf8ff04fcc9bf7c337533adb31,
title = "Slow and steady wins the race: physical limits on the rate of viral DNA packaging",
abstract = "During the assembly of dsDNA viruses such as the tailed bacteriophages and herpesviruses, the viral chromosome is compacted to near crystalline density inside a preformed head shell. DNA translocation is driven by powerful ring ATPase motors that couple ATP binding, hydrolysis, and release to force generation and movement. Studies of the motor of the bacteriophage phi29 have revealed a complex mechanochemistry behind this process that slows as the head fills. Recent studies of the physical behavior of packaging DNA suggest that surprisingly long-time scales of relaxation of DNA inside the head and jamming phenomena during packaging create the physical need for regulation of the rate of packaging. Studies of DNA packaging in viral systems have, therefore, revealed fundamental insight into the complex behavior of DNA and the need for biological systems to accommodate these physical constraints.",
author = "Jardine, {Paul J}",
year = "2019",
month = "6",
doi = "10.1016/j.coviro.2019.03.002",
language = "English (US)",
volume = "36",
pages = "32--37",
journal = "Current Opinion in Virology",
issn = "1879-6257",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Slow and steady wins the race

T2 - physical limits on the rate of viral DNA packaging

AU - Jardine, Paul J

PY - 2019/6

Y1 - 2019/6

N2 - During the assembly of dsDNA viruses such as the tailed bacteriophages and herpesviruses, the viral chromosome is compacted to near crystalline density inside a preformed head shell. DNA translocation is driven by powerful ring ATPase motors that couple ATP binding, hydrolysis, and release to force generation and movement. Studies of the motor of the bacteriophage phi29 have revealed a complex mechanochemistry behind this process that slows as the head fills. Recent studies of the physical behavior of packaging DNA suggest that surprisingly long-time scales of relaxation of DNA inside the head and jamming phenomena during packaging create the physical need for regulation of the rate of packaging. Studies of DNA packaging in viral systems have, therefore, revealed fundamental insight into the complex behavior of DNA and the need for biological systems to accommodate these physical constraints.

AB - During the assembly of dsDNA viruses such as the tailed bacteriophages and herpesviruses, the viral chromosome is compacted to near crystalline density inside a preformed head shell. DNA translocation is driven by powerful ring ATPase motors that couple ATP binding, hydrolysis, and release to force generation and movement. Studies of the motor of the bacteriophage phi29 have revealed a complex mechanochemistry behind this process that slows as the head fills. Recent studies of the physical behavior of packaging DNA suggest that surprisingly long-time scales of relaxation of DNA inside the head and jamming phenomena during packaging create the physical need for regulation of the rate of packaging. Studies of DNA packaging in viral systems have, therefore, revealed fundamental insight into the complex behavior of DNA and the need for biological systems to accommodate these physical constraints.

UR - http://www.scopus.com/inward/record.url?scp=85064190557&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85064190557&partnerID=8YFLogxK

U2 - 10.1016/j.coviro.2019.03.002

DO - 10.1016/j.coviro.2019.03.002

M3 - Review article

C2 - 31003199

AN - SCOPUS:85064190557

VL - 36

SP - 32

EP - 37

JO - Current Opinion in Virology

JF - Current Opinion in Virology

SN - 1879-6257

ER -