Slc43a3 is a regulator of free fatty acid flux

Kathrin B. Hasbargen, Wen Jun Shen, Yiqiang Zhang, Xiaoming Hou, Wei Wang, Qui Shuo, David A. Bernlohr, Salman Azhar, Fredric B. Kraemer

Research output: Contribution to journalArticle

Abstract

Adipocytes take up long chain FAs through diffusion and protein-mediated transport, whereas FA efflux is considered to occur by diffusion. To identify potential membrane proteins that are involved in regulating FA flux in adipocytes, the expression levels of 55 membrane transporters without known function were screened in subcutaneous adipose samples from obese patients before and after bariatric surgery using branched DNA methodology. Among the 33 solute carrier (SLC) transporter family members screened, the expression of 14 members showed significant changes before and after bariatric surgery. One of them, Slc43a3, increased about 2.5-fold after bariatric surgery. Further investigation demonstrated that Slc43a3 is highly expressed in murine adipose tissue and induced during adipocyte differentiation in primary preadipocytes and in OP9 cells. Knockdown of Slc43a3 with siRNA in differentiated OP9 adipocytes reduced both basal and forskolin-stimulated FA efflux, while also increasing FA uptake and lipid droplet accumulation. In contrast, overexpression of Slc43a3 decreased FA uptake in differentiated OP9 cells and resulted in decreased lipid droplet accumulation. Therefore, Slc43a3 seems to regulate FA flux in adipocytes, functioning as a positive regulator of FA efflux and as a negative regulator of FA uptake.

Original languageEnglish (US)
Pages (from-to)734-745
Number of pages12
JournalJournal of lipid research
Volume61
Issue number5
DOIs
StatePublished - May 1 2020

Keywords

  • Adipose metabolism
  • Fatty acid efflux
  • Fatty acid uptake
  • Lipid droplet
  • Membrane transporters
  • Solute carrier family 43 member 3

PubMed: MeSH publication types

  • Journal Article

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  • Cite this

    Hasbargen, K. B., Shen, W. J., Zhang, Y., Hou, X., Wang, W., Shuo, Q., Bernlohr, D. A., Azhar, S., & Kraemer, F. B. (2020). Slc43a3 is a regulator of free fatty acid flux. Journal of lipid research, 61(5), 734-745. https://doi.org/10.1194/jlr.RA119000294