Skin graft survival in genetically identical cloned pigs

Michael J. Martin, John S. Logan, Dengping Yin, Connie Adams, Jeffrey Houtz, Jikun Shen, Anita S. Chong, Ajay Sharma, Guerard W. Byrne, Barry S. Wiseman

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Nuclear transfer technology allows for the reprogramming of somatic cells, and the production of embryonic stem cells and animals that are genetically identical in terms of nuclear DNA to the parental somatic cell. It is assumed that these products of nuclear transfer technology will be immunologically compatible to each other in spite of the fact that there are data that show differences in the expression patterns and phenotypes between animals produced by nuclear transfer. We have produced a series of cloned pigs from embryonic fibroblasts. Microsatellite analysis was used to confirm that the clones were genetically identical. Skin transplants were performed to assess immunological reactivity. Skin transplants between genetically identical cloned pigs were accepted, whereas third party grafts were rejected. Histological analysis of the grafts showed edema and mononuclear cell infiltrates in the recipient's skin in rejected grafts and not in grafts that were accepted. Our data supports the notion that genetically identical cloned pigs are immunologically compatible.

Original languageEnglish (US)
Pages (from-to)117-121
Number of pages5
JournalCloning and Stem Cells
Volume5
Issue number2
DOIs
StatePublished - Jan 1 2003
Externally publishedYes

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    Martin, M. J., Logan, J. S., Yin, D., Adams, C., Houtz, J., Shen, J., Chong, A. S., Sharma, A., Byrne, G. W., & Wiseman, B. S. (2003). Skin graft survival in genetically identical cloned pigs. Cloning and Stem Cells, 5(2), 117-121. https://doi.org/10.1089/153623003322234713