Chan et al. show that functional skeletal muscle stem cells can be produced from mouse pluripotent stem cells without genetic modification through teratoma formation. As few as 40,000 teratoma-derived cells can regenerate 80% of total muscle volume, improve force generation, and mature into functional muscle stem cells in vivo.
Bibliographical noteFunding Information:
The authors would like to thank Jinjoo Kang, Olivia Recht, and Cara-Lin Lonetree for their help with genotyping and animal husbandry. The monoclonal antibodies to PAX7, embryonic MHC, MHC, MHC-I, MHC-IIa, and MHC-IIb were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa. Plasmids pMXs-Oct3/4, pMXs-Sox2, and pMXs-Klf4 were gifts from Shinya Yamanaka via Addgene. The study was supported by National Institute of Neurological Disorders and Stroke (NINDS) grant R01 NS083549 (to M.K.), National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) grant R01 AR055299 (to R.C.R.P.), Regenerative Medicine Minnesota discovery science grant RMM 102516 001 (to S.S.-K.C.), and the Greg Marzolf Jr. Foundation (2016.MK).
- muscle stem cells
- pluripotent stem cells
- satellite cells