Sizing lipid droplets from adult and geriatric mouse liver tissue via nanoparticle tracking analysis

Katherine A. Muratore, Charles P. Najt, Nicholas M Livezey, James Marti, Douglas Mashek, Edgar Arriaga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The significance of lipid droplets in lipid metabolism, cell signaling, and regulating longevity is increasingly recognized, yet the lipid droplet’s unique properties and architecture make it difficult to size and study using conventional methods. To begin to address this issue, we demonstrate the capabilities of nanoparticle tracking analysis (NTA) for sizing of lipid droplets. NTA was found to be adequate to assess lipid droplet stability over time, indicating that lipid droplet preparations are stable for up to 24 h. NTA had the ability to compare the size distributions of lipid droplets from adult and geriatric mouse liver tissue, suggesting an age-related decrease in lipid droplet size. This is the first report on the use of NTA to size intracellular organelles.

Original languageEnglish (US)
Pages (from-to)3629-3638
Number of pages10
JournalAnalytical and Bioanalytical Chemistry
Volume410
Issue number16
DOIs
StatePublished - Jun 1 2018

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Geriatrics
Liver
Nanoparticles
Tissue
Lipids
Organelle Size
Cell signaling
Lipid Droplets
Lipid Metabolism

Keywords

  • Aging
  • Lipid droplets
  • Liver
  • Nanoparticle tracking analysis
  • Organelles

PubMed: MeSH publication types

  • Journal Article

Cite this

Sizing lipid droplets from adult and geriatric mouse liver tissue via nanoparticle tracking analysis. / Muratore, Katherine A.; Najt, Charles P.; Livezey, Nicholas M; Marti, James; Mashek, Douglas; Arriaga, Edgar.

In: Analytical and Bioanalytical Chemistry, Vol. 410, No. 16, 01.06.2018, p. 3629-3638.

Research output: Contribution to journalArticle

Muratore, Katherine A. ; Najt, Charles P. ; Livezey, Nicholas M ; Marti, James ; Mashek, Douglas ; Arriaga, Edgar. / Sizing lipid droplets from adult and geriatric mouse liver tissue via nanoparticle tracking analysis. In: Analytical and Bioanalytical Chemistry. 2018 ; Vol. 410, No. 16. pp. 3629-3638.
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