Abstract
Initiator nucleotides consisting of an anthracene ring system coupled to the 5'-phosphate of guanosine monophosphate via a polyethylene glycol (PEG) linker were chemically synthesized. When added to transcription reactions, these initiators were site-specifically incorporated into RNA transcripts yielding RNA conjugates with a fluorophore selectively attached to the 5'- end via a flexible linker. Besides sensitive fluorescence detection, the anthracene allowed the convenient modification with maleimides by Dieis- Alder reaction.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 7729-7732 |
| Number of pages | 4 |
| Journal | Tetrahedron Letters |
| Volume | 38 |
| Issue number | 44 |
| DOIs | |
| State | Published - Nov 3 1997 |
Bibliographical note
Funding Information:Acknowledgements: This work was supported by the Deutsche Forschungsgemeinschaft (grants # Ja 794/1-1 and SFB 344, TP B10 to A.J.). We gratefully acknowledge generous support by Prof. Dr. V.A. Erdmann, and spectrometric measurements performed by Drs. P. Franke, A. Sch/ifer, E. Nordhoffand S. Hahner.