Site-specific methionine oxidation initiates calmodulin degradation by the 20S proteasome

Edward M. Balog, Elizabeth L. Lockamy, David D Thomas, Deborah A Ferrington

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The proteasome is a key intracellular protease that regulates processes, such as signal transduction and protein quality control, through the selective degradation of specific proteins. Signals that target a protein for degradation, collectively known as degrons, have been defined for many proteins involved in cell signaling. However, the molecular signals involved in recognition and degradation of proteins damaged by oxidation have not been completely defined. The current study used biochemical and spectroscopic measurements to define the properties in calmodulin that initiate degradation by the 20S proteasome. Our experimental approach involved the generation of multiple calmodulin mutants with specific Met replaced by Leu. This strategy of site-directed mutagenesis permitted site-selective oxidation of Met to Met sulfoxide. We found that the oxidation-induced loss of secondary structure, as measured by circular dichroism, correlated with the rate of degradation for wild-type and mutants containing Leu substitutions in the C-terminus. However, no degradation was observed for mutants with Met to Leu substitution in the N-terminus, suggesting that oxidation-induced structural unfolding in the N-terminal region is essential for degradation by the 20S proteasome. Experiments comparing the thermodynamic stability of CaM mutants helped to further localize the critical site of oxidation-induced focal disruption between residues 51 and 72 in the N-terminal region. This work brings new biochemical and structural clarity to the concept of the degron, the portion of a protein that determines its susceptibility to degradation by the proteasome.

Original languageEnglish (US)
Pages (from-to)3005-3016
Number of pages12
JournalBiochemistry
Volume48
Issue number13
DOIs
StatePublished - Apr 7 2009

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Proteasome Endopeptidase Complex
Calmodulin
Methionine
Proteolysis
Degradation
Oxidation
sulfoxide
Proteins
Circular Dichroism
Site-Directed Mutagenesis
Thermodynamics
Quality Control
Signal Transduction
Peptide Hydrolases
Substitution reactions
Cell signaling
Signal transduction
Mutagenesis
Quality control
Thermodynamic stability

Cite this

Site-specific methionine oxidation initiates calmodulin degradation by the 20S proteasome. / Balog, Edward M.; Lockamy, Elizabeth L.; Thomas, David D; Ferrington, Deborah A.

In: Biochemistry, Vol. 48, No. 13, 07.04.2009, p. 3005-3016.

Research output: Contribution to journalArticle

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