Site-specific cleavage of HCV genomic RNA and its cloned core and NS5B genes by DNAzyme

Deepak Kumar, Indrajit Chaudhury, Premashis Kar, Rakha H. Das

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Background and Aims: The 9600 nt hepatitis C virus (HCV) genomic RNA has only one internal ribosome entry site (IRES) for translation to a single polyprotein. In search of nucleic acid-based antiviral agents, two 10-23 DNAzymes were designed to cleave the RNA in IRES and RNA dependent RNA polymerase (RDRP/NS5B) regions to prevent translation and replication of HCV RNA. Methods: In vitro cleavage of HCV RNA by IRES specific DNAzyme, CDz and NS5B specific DNAzyme, NDz was carried out using HCV genomic RNA and in vitro synthesized runoff transcripts of core and NS5B genes. Cleavage of core and NS5B mRNAs by DNAzyme (Dz) in HepG2 cells was assessed by reverse transcription polymerase chain reaction (RT-PCR) using RNA from cells co-transfected with cloned core or NS5B gene and its respective DNAzyme. Suppression of core or NS5B protein expression due to mRNA cleavage by Dz in co-transfected cells was determined by Western blot analysis and fluorescence intensity of fluorescent-tagged expressed protein. Reduction of NS5B protein activity in NDz co-transfected cells was determined by enzymatic assays. Results: The designed CDz and NDz cleaved HCV genomic RNA and their respective in vitro generated transcripts. Both mRNA and protein expressions of core or NS5B from their cloned genes reduced substantially when co-transfected with respective Dz. Reduction of RDRP expression by NDz was accompanied with its reduced enzyme activity. Increased RNA cleavage, inhibition of protein expression, and reduction of RDRP activity were observed on increasing Dz concentration. Conclusion: Core and NS5B targeted DNAzymes can be used in controlling the replication of HCV RNA.

Original languageEnglish (US)
Pages (from-to)872-878
Number of pages7
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume24
Issue number5
DOIs
StatePublished - May 2009

Keywords

  • Gene expression
  • HCV treatment
  • Viral hepatitis
  • Virology

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