Site directed mutagenesis: a tool for enzyme mechanism dissection

C. R. Wagner, S. J. Benkovic

Research output: Contribution to journalArticle

35 Scopus citations


Protein engineering has become the principle means of examining the active site of an enzyme to identify and quantify the roles of specific residues in ligand binding, specificity and catalysis. Site-specific mutagenesis has extended our knowledge gained from X-ray crystallography, and has provided striking proof that the intricate active-site geometry is supported by the remainder of the protein infrastructure for maximum catalytic efficiency.

Original languageEnglish (US)
Pages (from-to)263-270
Number of pages8
JournalTrends in biotechnology
Issue numberC
StatePublished - 1990
Externally publishedYes


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