siRNA-Mediated suppression of collagen type iv alpha 2 (COL4A2) mRNA inhibits triple-negative breast cancer cell proliferation and migration

He JingSong, Guan Hong, Jianbo Yang, Zheng Duo, Fu Li, Chen WeiCai, Luo XueYing, Mao YouSheng, Ou Yang YiWen, Pan Yue, Chang Zou

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Triple-negative breast cancer (TNBC) is more aggressive than other breast cancer subtypes. Collagen type IV alpha 2 (COL4A2), a major component of the basement membrane, dynamically influences a wide range of biological processes, including cancer pathogenesis and progression. This study evaluated the effects of COL4A2 siRNA delivered by lentiviral vector to TNBC cells. COL4A2 siRNA lenti-viral vector was constructed and transfected into MDA-MB-231 and MDA-MB-468 cells. The COL4A2 mRNA levels were quantified by RT-PCR. CCK8 assay was performed to evaluate cell proliferation and migration. Cell migration and invasion assays were carried out using Transwell. Cell apoptosis and cell cycle analyses were conducted using flow cytometric approach. We found that COL4A2 mRNA levels were significantly down-regulated in MDA-MB-231 and MDA-MB-468 cells after transfection with COL4A2 siRNA. Furthermore, cell migration and proliferation were significantly decreased and the cell cycle was arrested. Our results indicated that COL4A2 siRNA significantly suppresses the migration and proliferation of TNBC cells. Inhibition of COL4A2 may be a new target for the prevention and treatment of TNBC.

Original languageEnglish (US)
Pages (from-to)2585-2593
Number of pages9
JournalOncotarget
Volume8
Issue number2
DOIs
StatePublished - 2017

Bibliographical note

Funding Information:
This work were supported by the National Natural Science Foundation of China (81372583 and 81402370), The Scientific and Technology Foundation of Guangdong Province (2015B090904007), The Natural Science Foundation of Guangdong Province (2015A030313829), the Science and Technology Foundation of Shenzhen (CXZZ20150430092951135, KQTD20140630100658078, and JCYJ20150422150029094), Shenzhen city science and technology innovation international cooperation projects 2014 (NO.GJHZ20140414170821180) ,the Natural Science Foundation of SZU (201573), and the Key Laboratory Project of Shenzhen (ZDSY20130329101130496).

Keywords

  • COL4A2
  • Lentiviral vector
  • Small interfering RNA (siRNA)
  • Triple-negative breast cancer (TNBC)

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