Single-stranded conformational polymorphism for separation of mixed rRNAS (rRNA-SSCP), a new method for profiling microbial communities

Medicine & Life Sciences

• 16S Ribosomal RNA 38%
• Clone Cells 29%
• Digestion 33%
• Electrophoresis 35%
• Gels 60%
• Microbiota 73%
• Oligonucleotides 38%
• Organism Cloning 33%
• Polymerase Chain Reaction 43%
• Restriction Fragment Length Polymorphisms 36%
• Ribonuclease H 53%
• Ribosomes 38%
• RNA 25%
• Single-Stranded Conformational Polymorphism 100%

Earth & Environmental Sciences

• amplification 23%
• clone 26%
• cloning 30%
• community composition 22%
• digestion 23%
• electrokinesis 23%
• gel 41%
• incorporation 20%
• library 22%
• method 16%
• microbial community 61%
• microbial mat 30%
• polymorphism 77%
• RNA 25%
• substrate 16%

Agriculture & Biology

• autoradiography 18%
• clones 12%
• digestion 13%
• gel electrophoresis 16%
• gels 12%
• hybridization 14%
• methodology 19%
• microbial communities 70%
• molecular cloning 13%
• oligonucleotides 18%
• restriction fragment length polymorphism 16%
• reverse transcriptase polymerase chain reaction 14%
• ribonucleases 20%
• ribosomal RNA 52%
• ribosomes 17%
• RNA 12%
• sampling 6%
• single-stranded conformational polymorphism 92%