Single nucleotide polymorphisms for integrative mapping in the Turkey (Meleagris gallopavo)

K. M. Reed, M. K. Hall, L. D. Chaves, T. P. Knutson

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

When multiple genetic maps exist for a species, integration of these maps requires a set of common markers be genotyped across the individual mapping populations. In the turkey, three genetic maps based on separate mapping populations are available. In this study, SNP-based markers were developed for integrating the cDNAIRFLP-based map (1) with microsatellite markers of the second-generation turkey genome map (2). Forty-eight primer sets were designed and tested and 33 (69%) correctly amplified turkey genomic DNA by PCR. Putative SNPs were detected in 20 (61%) of the amplified gene fragments, and 10 SNP markers were subsequently genotyped by PCRIRFLP for segregation analysis. Eight SNP markers were incorporated into the turkey genetic map.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalAnimal Biotechnology
Volume17
Issue number1
DOIs
StatePublished - May 1 2006

Keywords

  • Genetic linkage map
  • Single nucleotide polymorphism
  • Turkey

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