Asymmetric distribution of mRNA is a prevalent phenomenon observed in diverse cell types. The posttranscriptional movement and localization of mRNA provides an important mechanism to target certain proteins to specific cytoplasmic regions of their function. Recent technical advances have enabled real-time visualization of single mRNA molecules in living cells. Studies analyzing the motion of individual mRNAs have shed light on the complex RNA transport system. This chapter presents an overview of general approaches for single particle tracking and some methodologies that are used for single mRNA detection.
|Original language||English (US)|
|Title of host publication||Methods in Enzymology|
|Publisher||Academic Press Inc.|
|Number of pages||20|
|State||Published - Jan 2010|
|Name||Methods in Enzymology|
Bibliographical noteFunding Information:
This work was supported by National Institutes of Health grant EB2060. H. Y. P. was also supported by National Research Service Awards 5T32 HL007675 and 1F32 GM087122.
© 2010 Elsevier Inc.