Single mRNA Tracking in Live Cells

Hye Yoon Park; Adina R. Buxbaum; Robert H. Singer

doi:10.1016/S0076-6879(10)72003-6

Single mRNA Tracking in Live Cells

Hye Yoon Park, Adina R. Buxbaum, Robert H. Singer

Research output: Chapter in Book/Report/Conference proceeding › Chapter

62 Scopus citations

Abstract

Asymmetric distribution of mRNA is a prevalent phenomenon observed in diverse cell types. The posttranscriptional movement and localization of mRNA provides an important mechanism to target certain proteins to specific cytoplasmic regions of their function. Recent technical advances have enabled real-time visualization of single mRNA molecules in living cells. Studies analyzing the motion of individual mRNAs have shed light on the complex RNA transport system. This chapter presents an overview of general approaches for single particle tracking and some methodologies that are used for single mRNA detection.

Original language	English (US)
Title of host publication	Methods in Enzymology
Publisher	Academic Press Inc.
Pages	387-406
Number of pages	20
DOIs	https://doi.org/10.1016/S0076-6879(10)72003-6
State	Published - Jan 2010
Externally published	Yes

Publication series

Name	Methods in Enzymology
Volume	472
ISSN (Print)	0076-6879
ISSN (Electronic)	1557-7988

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Publisher Copyright:
© 2010 Elsevier Inc.

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10.1016/S0076-6879(10)72003-6

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abstract = "Asymmetric distribution of mRNA is a prevalent phenomenon observed in diverse cell types. The posttranscriptional movement and localization of mRNA provides an important mechanism to target certain proteins to specific cytoplasmic regions of their function. Recent technical advances have enabled real-time visualization of single mRNA molecules in living cells. Studies analyzing the motion of individual mRNAs have shed light on the complex RNA transport system. This chapter presents an overview of general approaches for single particle tracking and some methodologies that are used for single mRNA detection.",

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AU - Buxbaum, Adina R.

AU - Singer, Robert H.

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AB - Asymmetric distribution of mRNA is a prevalent phenomenon observed in diverse cell types. The posttranscriptional movement and localization of mRNA provides an important mechanism to target certain proteins to specific cytoplasmic regions of their function. Recent technical advances have enabled real-time visualization of single mRNA molecules in living cells. Studies analyzing the motion of individual mRNAs have shed light on the complex RNA transport system. This chapter presents an overview of general approaches for single particle tracking and some methodologies that are used for single mRNA detection.

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M3 - Chapter

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T3 - Methods in Enzymology

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BT - Methods in Enzymology

PB - Academic Press Inc.

ER -

Single mRNA Tracking in Live Cells

Abstract

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