Single-mRNA counting using fluorescent in situ hybridization in budding yeast

Keyphrases

• Budding Yeast 100%
• Fluorescence in Situ Hybridization 100%
• Detection Algorithm 40%
• Spot Detection 40%
• Wide-field 20%
• Spatial Distribution 20%
• Coverslip 20%
• Individual Cells 20%
• Epifluorescence Microscopy 20%
• Quantitative PCR 20%
• High-throughput Sequencing 20%
• PCR Array 20%
• Yeast Cells 20%
• Single-stranded DNA (ssDNA) 20%
• DNA Probe 20%
• Fluorescent Labeling 20%
• Diffraction Limit 20%
• Hybridization Probes 20%
• Clonal Population 20%
• Fluorescent Dye 20%
• Phenotypic Differences 20%
• RNA Content 20%
• Image Acquisition 20%
• Cellular RNA 20%
• Stochastic Fluctuations 20%
• In(III) 20%
• Hybridisation Procedure 20%
• Single-molecule Analysis 20%

Biochemistry, Genetics and Molecular Biology

• Budding Yeast 100%
• Fluorescence in Situ Hybridization 100%
• Messenger RNA 100%
• RNA 40%
• DNA Probe 20%
• Single-Stranded DNA 20%
• Clonal Population 20%
• High Throughput Sequencing 20%
• Hybridization Probe 20%
• Reverse Transcription Polymerase Chain Reaction 20%
• Diffraction 20%