Single-Molecule Dual-Beam Optical Trap Analysis of Protein Structure and Function

Jongmin Sung, Sivaraj Sivaramakrishnan, Alexander R. Dunn, James A. Spudich

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Optical trapping is one of the most powerful single-molecule techniques. We provide a practical guide to set up and use an optical trap, applied to the molecular motor myosin as an example. We focus primarily on studies of myosin function using a dual-beam optical trap, a protocol to build such a trap, and the experimental and data analysis protocols to utilize it.

Original languageEnglish (US)
Pages (from-to)321-375
Number of pages55
JournalMethods in Enzymology
Volume475
Issue numberC
DOIs
StatePublished - Jan 1 2010

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