Single intravenous injection of plasmid DNA encoding human paraoxonase-1 inhibits hyperlipidemia in rats

Ai Ling Fu; Shao Ping Wu

doi:10.1016/j.bbrc.2010.05.095

Single intravenous injection of plasmid DNA encoding human paraoxonase-1 inhibits hyperlipidemia in rats

Ai Ling Fu
, Shao Ping Wu

Neurosurgery

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Paraoxonase-1 (PON1, EC 3.1.8.1) is a high-density lipoprotein (HDL)-associated antioxidant enzyme, and its activity correlates negatively with the level of plasma low-density lipoprotein cholesterol (LDL-C) and triglyceridemia (TG). In this study, we examined the therapeutic effect of plasmid DNA containing the human PON1 gene (pcDNA/PON1) in hyperlipidemic model rats. The rats were fed a high-fat and highcholesterol diet for 25 days to produce a hyperlipidemic animal model. Single intravenous injection of pcDNA/PON1 into model rats prevented dyslipidemia and hepatic lipid accumulation. The mechanisms of pcDNA/PON1 in treating hyperlipidemia were associated with increases of serum antioxidant PON1 and SOD activities, and with reduction of the levels of total cholesterol (TC), LDL-C and TG. The results suggest the potential therapeutic effect of pcDNA/PON1 on hyperlipidemia.

Original language	English (US)
Pages (from-to)	257-262
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	397
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2010.05.095
State	Published - Jun 25 2010

Keywords

Antioxidant enzyme
Gene therapy
Hyperlipidemia
Paraoxonase-1

Publisher link

10.1016/j.bbrc.2010.05.095

Find It

Find It at U of M Libraries

Cite this

@article{22c9308f35bc463e8aad13c918684f35,

title = "Single intravenous injection of plasmid DNA encoding human paraoxonase-1 inhibits hyperlipidemia in rats",

abstract = "Paraoxonase-1 (PON1, EC 3.1.8.1) is a high-density lipoprotein (HDL)-associated antioxidant enzyme, and its activity correlates negatively with the level of plasma low-density lipoprotein cholesterol (LDL-C) and triglyceridemia (TG). In this study, we examined the therapeutic effect of plasmid DNA containing the human PON1 gene (pcDNA/PON1) in hyperlipidemic model rats. The rats were fed a high-fat and highcholesterol diet for 25 days to produce a hyperlipidemic animal model. Single intravenous injection of pcDNA/PON1 into model rats prevented dyslipidemia and hepatic lipid accumulation. The mechanisms of pcDNA/PON1 in treating hyperlipidemia were associated with increases of serum antioxidant PON1 and SOD activities, and with reduction of the levels of total cholesterol (TC), LDL-C and TG. The results suggest the potential therapeutic effect of pcDNA/PON1 on hyperlipidemia.",

keywords = "Antioxidant enzyme, Gene therapy, Hyperlipidemia, Paraoxonase-1",

author = "Fu, \{Ai Ling\} and Wu, \{Shao Ping\}",

year = "2010",

month = jun,

day = "25",

doi = "10.1016/j.bbrc.2010.05.095",

language = "English (US)",

volume = "397",

pages = "257--262",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Single intravenous injection of plasmid DNA encoding human paraoxonase-1 inhibits hyperlipidemia in rats

AU - Fu, Ai Ling

AU - Wu, Shao Ping

PY - 2010/6/25

Y1 - 2010/6/25

N2 - Paraoxonase-1 (PON1, EC 3.1.8.1) is a high-density lipoprotein (HDL)-associated antioxidant enzyme, and its activity correlates negatively with the level of plasma low-density lipoprotein cholesterol (LDL-C) and triglyceridemia (TG). In this study, we examined the therapeutic effect of plasmid DNA containing the human PON1 gene (pcDNA/PON1) in hyperlipidemic model rats. The rats were fed a high-fat and highcholesterol diet for 25 days to produce a hyperlipidemic animal model. Single intravenous injection of pcDNA/PON1 into model rats prevented dyslipidemia and hepatic lipid accumulation. The mechanisms of pcDNA/PON1 in treating hyperlipidemia were associated with increases of serum antioxidant PON1 and SOD activities, and with reduction of the levels of total cholesterol (TC), LDL-C and TG. The results suggest the potential therapeutic effect of pcDNA/PON1 on hyperlipidemia.

AB - Paraoxonase-1 (PON1, EC 3.1.8.1) is a high-density lipoprotein (HDL)-associated antioxidant enzyme, and its activity correlates negatively with the level of plasma low-density lipoprotein cholesterol (LDL-C) and triglyceridemia (TG). In this study, we examined the therapeutic effect of plasmid DNA containing the human PON1 gene (pcDNA/PON1) in hyperlipidemic model rats. The rats were fed a high-fat and highcholesterol diet for 25 days to produce a hyperlipidemic animal model. Single intravenous injection of pcDNA/PON1 into model rats prevented dyslipidemia and hepatic lipid accumulation. The mechanisms of pcDNA/PON1 in treating hyperlipidemia were associated with increases of serum antioxidant PON1 and SOD activities, and with reduction of the levels of total cholesterol (TC), LDL-C and TG. The results suggest the potential therapeutic effect of pcDNA/PON1 on hyperlipidemia.

KW - Antioxidant enzyme

KW - Gene therapy

KW - Hyperlipidemia

KW - Paraoxonase-1

UR - https://www.scopus.com/pages/publications/77955497134

UR - https://www.scopus.com/pages/publications/77955497134#tab=citedBy

U2 - 10.1016/j.bbrc.2010.05.095

DO - 10.1016/j.bbrc.2010.05.095

M3 - Article

C2 - 20580687

AN - SCOPUS:77955497134

SN - 0006-291X

VL - 397

SP - 257

EP - 262

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Single intravenous injection of plasmid DNA encoding human paraoxonase-1 inhibits hyperlipidemia in rats

Abstract

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this