Single intravenous injection of plasmid DNA encoding human paraoxonase-1 inhibits hyperlipidemia in rats

Ai Ling Fu, Shao Ping Wu

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Paraoxonase-1 (PON1, EC 3.1.8.1) is a high-density lipoprotein (HDL)-associated antioxidant enzyme, and its activity correlates negatively with the level of plasma low-density lipoprotein cholesterol (LDL-C) and triglyceridemia (TG). In this study, we examined the therapeutic effect of plasmid DNA containing the human PON1 gene (pcDNA/PON1) in hyperlipidemic model rats. The rats were fed a high-fat and highcholesterol diet for 25 days to produce a hyperlipidemic animal model. Single intravenous injection of pcDNA/PON1 into model rats prevented dyslipidemia and hepatic lipid accumulation. The mechanisms of pcDNA/PON1 in treating hyperlipidemia were associated with increases of serum antioxidant PON1 and SOD activities, and with reduction of the levels of total cholesterol (TC), LDL-C and TG. The results suggest the potential therapeutic effect of pcDNA/PON1 on hyperlipidemia.

Original languageEnglish (US)
Pages (from-to)257-262
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume397
Issue number2
DOIs
StatePublished - Jun 25 2010

Keywords

  • Antioxidant enzyme
  • Gene therapy
  • Hyperlipidemia
  • Paraoxonase-1

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