Abstract
Paraoxonase-1 (PON1, EC 3.1.8.1) is a high-density lipoprotein (HDL)-associated antioxidant enzyme, and its activity correlates negatively with the level of plasma low-density lipoprotein cholesterol (LDL-C) and triglyceridemia (TG). In this study, we examined the therapeutic effect of plasmid DNA containing the human PON1 gene (pcDNA/PON1) in hyperlipidemic model rats. The rats were fed a high-fat and highcholesterol diet for 25 days to produce a hyperlipidemic animal model. Single intravenous injection of pcDNA/PON1 into model rats prevented dyslipidemia and hepatic lipid accumulation. The mechanisms of pcDNA/PON1 in treating hyperlipidemia were associated with increases of serum antioxidant PON1 and SOD activities, and with reduction of the levels of total cholesterol (TC), LDL-C and TG. The results suggest the potential therapeutic effect of pcDNA/PON1 on hyperlipidemia.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 257-262 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 397 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 25 2010 |
Keywords
- Antioxidant enzyme
- Gene therapy
- Hyperlipidemia
- Paraoxonase-1