Single-Digit Pathogen and Attomolar Detection with the Naked Eye Using Liposome-Amplified Plasmonic Immunoassay

Minh Phuong Ngoc Bui; Snober Ahmed; Abdennour Abbas

doi:10.1021/acs.nanolett.5b02837

Single-Digit Pathogen and Attomolar Detection with the Naked Eye Using Liposome-Amplified Plasmonic Immunoassay

Minh Phuong Ngoc Bui, Snober Ahmed, Abdennour Abbas

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

91 Scopus citations

Abstract

We introduce an enzyme-free plasmonic immunoassay with a binary (all-or-none) response. The presence of a single pathogen in the sample results in a chemical cascade reaction leading to a large red to dark-blue colorimetric shift visible to the naked eye. The immediate and amplified response is initiated by a triggered breakdown of cysteine-loaded nanoliposomes and subsequent aggregation of plasmonic gold nanoparticles. Our approach enabled visual detection of a single-digit live pathogen of Salmonella, Listeria, and E. coli O157 in water and food samples. Furthermore, the assay allowed a naked-eye detection of target antibody concentrations as low as 6.7 attomolar (600 molecules in 150 μL); six orders of magnitude lower than conventional enzyme-linked immunosorbent assay (ELISA).

Original language	English (US)
Pages (from-to)	6239-6246
Number of pages	8
Journal	Nano letters
Volume	15
Issue number	9
DOIs	https://doi.org/10.1021/acs.nanolett.5b02837
State	Published - Sep 9 2015

Keywords

Plasmonic colorimetry
foodborne pathogens
gold nanoparticles
immunoassay
liposomes
signal amplification

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10.1021/acs.nanolett.5b02837

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title = "Single-Digit Pathogen and Attomolar Detection with the Naked Eye Using Liposome-Amplified Plasmonic Immunoassay",

abstract = "We introduce an enzyme-free plasmonic immunoassay with a binary (all-or-none) response. The presence of a single pathogen in the sample results in a chemical cascade reaction leading to a large red to dark-blue colorimetric shift visible to the naked eye. The immediate and amplified response is initiated by a triggered breakdown of cysteine-loaded nanoliposomes and subsequent aggregation of plasmonic gold nanoparticles. Our approach enabled visual detection of a single-digit live pathogen of Salmonella, Listeria, and E. coli O157 in water and food samples. Furthermore, the assay allowed a naked-eye detection of target antibody concentrations as low as 6.7 attomolar (600 molecules in 150 μL); six orders of magnitude lower than conventional enzyme-linked immunosorbent assay (ELISA).",

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