Single Cell RNA Sequencing in Atherosclerosis Research

Jesse W. Williams, Holger Winkels, Christopher P. Durant, Konstantin Zaitsev, Yanal Ghosheh, Klaus Ley

Research output: Contribution to journalArticlepeer-review

74 Scopus citations


Technological advances in characterizing molecular heterogeneity at the single cell level have ushered in a deeper understanding of the biological diversity of cells present in tissues including atherosclerotic plaques. New subsets of cells have been discovered among cell types previously considered homogenous. The commercial availability of systems to obtain transcriptomes and matching surface phenotypes from thousands of single cells is rapidly changing our understanding of cell types and lineage identity. Emerging methods to infer cellular functions are beginning to shed new light on the interplay of components involved in multifaceted disease responses, like atherosclerosis. Here, we provide a technical guide for design, implementation, assembly, and interpretations of current single cell transcriptomics approaches from the perspective of employing these tools for advancing cardiovascular disease research.

Original languageEnglish (US)
Pages (from-to)1112-1126
Number of pages15
JournalCirculation research
Issue number9
StatePublished - Apr 24 2020

Bibliographical note

Publisher Copyright:
© 2021 American Heart Association, Inc.


  • atherosclerosis
  • cardiovascular disease
  • immunology
  • phenotype
  • transcriptomic


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