Single-cell RNA-seq analysis of Mesp1-induced skeletal myogenic development

Jacqueline S Penaloza; Matthew P. Pappas; Hannah R. Hagen; Ning Xie; Sunny S.K. Chan

doi:10.1016/j.bbrc.2019.09.140

Single-cell RNA-seq analysis of Mesp1-induced skeletal myogenic development

Jacqueline S Penaloza, Matthew P. Pappas, Hannah R. Hagen, Ning Xie, Sunny S.K. Chan

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The Mesp1 lineage contributes to cardiac, hematopoietic and skeletal myogenic development. Interestingly, muscle stem cells residing in craniofacial skeletal muscles primarily arise from Mesp1+ progenitors, but those in trunk and limb skeletal muscles do not. To gain insights into the difference between the head and trunk/limb muscle developmental processes, we studied Mesp1+ skeletal myogenic derivatives via single-cell RNA-seq and other strategies. Using a doxycycline-inducible Mesp1-expressing mouse embryonic stem cell line, we found that the development of Mesp1-induced skeletal myogenic progenitors can be characterized by dynamic expression of PDGFRα and VCAM1. Single-cell RNA-seq analysis further revealed the heterogeneous nature of these Mesp1+ derivatives, spanning pluripotent and mesodermal to mesenchymal and skeletal myogenic. We subsequently reconstructed the single-cell trajectories of these subpopulations. Our data thereby provide a cell fate projection of Mesp1-induced skeletal myogenesis.

Original language	English (US)
Pages (from-to)	284-290
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	520
Issue number	2
DOIs	https://doi.org/10.1016/j.bbrc.2019.09.140
State	Published - Dec 3 2019

Bibliographical note

Funding Information:
We would like to thank Jinjoo Kang, Olivia Recht and Cara-Lin Lonetree for their help with genotyping and animal husbandry, and Dr. Micah Gearhart for his advice on scRNA-seq analysis. The monoclonal antibody to MHC and Myogenin were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa. This work is supported by Regenerative Medicine Minnesota Discovery Science Grant, Minnesota, United States (grant number RMM 102516 001, to S.S.K.C.) and by University of Minnesota start-up fund, Minnesota, United States (to S.S.K.C.).

Funding Information:
We would like to thank Jinjoo Kang, Olivia Recht and Cara-Lin Lonetree for their help with genotyping and animal husbandry, and Dr. Micah Gearhart for his advice on scRNA-seq analysis. The monoclonal antibody to MHC and Myogenin were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa. This work is supported by Regenerative Medicine Minnesota Discovery Science Grant, Minnesota, United States (grant number RMM 102516 001, to S.S.K.C. ) and by University of Minnesota start-up fund, Minnesota, United States (to S.S.K.C.).

Publisher Copyright:
© 2019 Elsevier Inc.

Keywords

Mesoderm patterning
Mesp1
Myogenic differentiation
Single-cell RNA-Sequencing
Skeletal muscle

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title = "Single-cell RNA-seq analysis of Mesp1-induced skeletal myogenic development",

abstract = "The Mesp1 lineage contributes to cardiac, hematopoietic and skeletal myogenic development. Interestingly, muscle stem cells residing in craniofacial skeletal muscles primarily arise from Mesp1+ progenitors, but those in trunk and limb skeletal muscles do not. To gain insights into the difference between the head and trunk/limb muscle developmental processes, we studied Mesp1+ skeletal myogenic derivatives via single-cell RNA-seq and other strategies. Using a doxycycline-inducible Mesp1-expressing mouse embryonic stem cell line, we found that the development of Mesp1-induced skeletal myogenic progenitors can be characterized by dynamic expression of PDGFRα and VCAM1. Single-cell RNA-seq analysis further revealed the heterogeneous nature of these Mesp1+ derivatives, spanning pluripotent and mesodermal to mesenchymal and skeletal myogenic. We subsequently reconstructed the single-cell trajectories of these subpopulations. Our data thereby provide a cell fate projection of Mesp1-induced skeletal myogenesis.",

keywords = "Mesoderm patterning, Mesp1, Myogenic differentiation, Single-cell RNA-Sequencing, Skeletal muscle",

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note = "Funding Information: We would like to thank Jinjoo Kang, Olivia Recht and Cara-Lin Lonetree for their help with genotyping and animal husbandry, and Dr. Micah Gearhart for his advice on scRNA-seq analysis. The monoclonal antibody to MHC and Myogenin were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa. This work is supported by Regenerative Medicine Minnesota Discovery Science Grant, Minnesota, United States (grant number RMM 102516 001, to S.S.K.C.) and by University of Minnesota start-up fund, Minnesota, United States (to S.S.K.C.). Funding Information: We would like to thank Jinjoo Kang, Olivia Recht and Cara-Lin Lonetree for their help with genotyping and animal husbandry, and Dr. Micah Gearhart for his advice on scRNA-seq analysis. The monoclonal antibody to MHC and Myogenin were obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by the University of Iowa. This work is supported by Regenerative Medicine Minnesota Discovery Science Grant, Minnesota, United States (grant number RMM 102516 001, to S.S.K.C. ) and by University of Minnesota start-up fund, Minnesota, United States (to S.S.K.C.). Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

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doi = "10.1016/j.bbrc.2019.09.140",

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AU - Hagen, Hannah R.

AU - Xie, Ning

AU - Chan, Sunny S.K.

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N2 - The Mesp1 lineage contributes to cardiac, hematopoietic and skeletal myogenic development. Interestingly, muscle stem cells residing in craniofacial skeletal muscles primarily arise from Mesp1+ progenitors, but those in trunk and limb skeletal muscles do not. To gain insights into the difference between the head and trunk/limb muscle developmental processes, we studied Mesp1+ skeletal myogenic derivatives via single-cell RNA-seq and other strategies. Using a doxycycline-inducible Mesp1-expressing mouse embryonic stem cell line, we found that the development of Mesp1-induced skeletal myogenic progenitors can be characterized by dynamic expression of PDGFRα and VCAM1. Single-cell RNA-seq analysis further revealed the heterogeneous nature of these Mesp1+ derivatives, spanning pluripotent and mesodermal to mesenchymal and skeletal myogenic. We subsequently reconstructed the single-cell trajectories of these subpopulations. Our data thereby provide a cell fate projection of Mesp1-induced skeletal myogenesis.

AB - The Mesp1 lineage contributes to cardiac, hematopoietic and skeletal myogenic development. Interestingly, muscle stem cells residing in craniofacial skeletal muscles primarily arise from Mesp1+ progenitors, but those in trunk and limb skeletal muscles do not. To gain insights into the difference between the head and trunk/limb muscle developmental processes, we studied Mesp1+ skeletal myogenic derivatives via single-cell RNA-seq and other strategies. Using a doxycycline-inducible Mesp1-expressing mouse embryonic stem cell line, we found that the development of Mesp1-induced skeletal myogenic progenitors can be characterized by dynamic expression of PDGFRα and VCAM1. Single-cell RNA-seq analysis further revealed the heterogeneous nature of these Mesp1+ derivatives, spanning pluripotent and mesodermal to mesenchymal and skeletal myogenic. We subsequently reconstructed the single-cell trajectories of these subpopulations. Our data thereby provide a cell fate projection of Mesp1-induced skeletal myogenesis.

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KW - Single-cell RNA-Sequencing

KW - Skeletal muscle

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Single-cell RNA-seq analysis of Mesp1-induced skeletal myogenic development

Abstract

Bibliographical note

Keywords

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