Abstract
Recent advancements in single-cell and single-molecule imaging technologies have resolved biological processes in time and space that are fundamental to understanding the regulation of gene expression. Observations of single-molecule events in their cellular context have revealed highly dynamic aspects of transcriptional and post-transcriptional control in eukaryotic cells. This approach can relate transcription with mRNA abundance and lifetimes. Another key aspect of single-cell analysis is the cell-to-cell variability among populations of cells. Definition of heterogeneity has revealed stochastic processes, determined characteristics of under-represented cell types or transitional states, and integrated cellular behaviors in the context of multicellular organisms. In this review, we discuss novel aspects of gene expression of eukaryotic cells and multicellular organisms revealed by the latest advances in single-cell and single-molecule imaging technology.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 267-291 |
| Number of pages | 25 |
| Journal | Annual Review of Genetics |
| Volume | 50 |
| DOIs | |
| State | Published - Nov 23 2016 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2016 by Annual Reviews. All rights reserved.
Keywords
- Gene expression
- Single cell
- Single-molecule imaging
- Transcription
- Translation
- mRNA localization
