Simultaneous production of triacylglycerol and high-value carotenoids by the astaxanthin-producing oleaginous green microalga Chlorella zofingiensis

Jin Liu, Xuemei Mao, Wenguang Zhou, Michael T. Guarnieri

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

The production of lipids and astaxanthin, a high-value carotenoid, by Chlorella zofingiensis was investigated under different culture conditions. Comparative analysis revealed a good correlation between triacylglycerol (TAG) and astaxanthin accumulation in C. zofingiensis. Stress conditions promoted cell size and weight and induced the accumulation of neutral lipids, especially TAG and astaxanthin, with a concomitant decrease in membrane lipids. The highest contents of TAG and astaxanthin achieved were 387 and 4.89 mg g-1 dry weight, respectively. A semi-continuous culture strategy was developed to optimize the TAG and astaxanthin productivities, which reached 297 and 3.3 mg L-1 day-1, respectively. Additionally, astaxanthin accumulation was enhanced by inhibiting de novo fatty acid biosynthesis. In summary, our study represents a pioneering work of utilizing Chlorella for the integrated production of lipids and high-value products and C. zofingiensis has great potential to be a promising production strain and serve as an emerging oleaginous model alga.

Original languageEnglish (US)
Pages (from-to)319-327
Number of pages9
JournalBioresource Technology
Volume214
DOIs
StatePublished - Aug 1 2016

Keywords

  • Astaxanthin
  • Biofuels
  • Chlorella zofingiensis
  • Integrated production
  • Stress conditions
  • Triacylglycerol

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