Simultaneous production of 1,3-dihydroxyacetone and xylitol from glycerol and xylose using a nanoparticle-supported multi-enzyme system with in situ cofactor regeneration

Ying Zhang, Fei Gao, Song Ping Zhang, Zhi Guo Su, Guang Hui Ma, Ping Wang

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

Cofactor-dependent biotransformations often require consumption of a secondary substrate for cofactor regeneration. Alternatively, two synthetic reactions may be coupled together through cofactor regeneration cycles. Simultaneous production of value-added products from glycerol and xylose was realized in this work through an enzymatic NAD(H) regeneration cycle involving two enzymes. Glycerol dehydrogenase (GDH) catalyzed the production of 1,3-dihydroxyacetone (DHA) from glycerol, while xylose reductase (XR) enabled the reduction of xylose to xylitol using the protons released from glycerol. Both enzymes were immobilized with P(MMA-EDMA-MAA) nanoparticles. Interestingly, the immobilized multi-enzyme system showed much improved productivity and stability as compared to native enzymes, such that the total turnover number (TTN) reached 82 for cofactor regeneration while the yield reached 160. g/g-immobilized GDH for DHA production.

Original languageEnglish (US)
Pages (from-to)1837-1843
Number of pages7
JournalBioresource Technology
Volume102
Issue number2
DOIs
StatePublished - Jan 2011

Bibliographical note

Funding Information:
The authors thank support from National Basic Research Program of China (973 Program, Contract No. 2009CB724705), Chinese Academy of Sciences (KSCX2-YW-G-019), and 863 Project (2008AA10Z302). Wang acknowledges the support from Chinese National Science Foundation of China for Distinguished Young Scholars (Abroad, #20728607). The authors thank Ms. Yufei Zhang and Mr. Yingfu Zheng for assistance in the production of xylose reductase.

Keywords

  • Biosynthesis
  • Cofactor regeneration
  • Enzymatic biocatalysis
  • Glycerol dehydrogenase
  • Xylose reductase

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