Abstract
Cofactor-dependent biotransformations often require consumption of a secondary substrate for cofactor regeneration. Alternatively, two synthetic reactions may be coupled together through cofactor regeneration cycles. Simultaneous production of value-added products from glycerol and xylose was realized in this work through an enzymatic NAD(H) regeneration cycle involving two enzymes. Glycerol dehydrogenase (GDH) catalyzed the production of 1,3-dihydroxyacetone (DHA) from glycerol, while xylose reductase (XR) enabled the reduction of xylose to xylitol using the protons released from glycerol. Both enzymes were immobilized with P(MMA-EDMA-MAA) nanoparticles. Interestingly, the immobilized multi-enzyme system showed much improved productivity and stability as compared to native enzymes, such that the total turnover number (TTN) reached 82 for cofactor regeneration while the yield reached 160. g/g-immobilized GDH for DHA production.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1837-1843 |
| Number of pages | 7 |
| Journal | Bioresource Technology |
| Volume | 102 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 2011 |
Bibliographical note
Funding Information:The authors thank support from National Basic Research Program of China (973 Program, Contract No. 2009CB724705), Chinese Academy of Sciences (KSCX2-YW-G-019), and 863 Project (2008AA10Z302). Wang acknowledges the support from Chinese National Science Foundation of China for Distinguished Young Scholars (Abroad, #20728607). The authors thank Ms. Yufei Zhang and Mr. Yingfu Zheng for assistance in the production of xylose reductase.
Keywords
- Biosynthesis
- Cofactor regeneration
- Enzymatic biocatalysis
- Glycerol dehydrogenase
- Xylose reductase