Salivary proteins have multiple functions and many share similar functions, which may be why it has been difficult to relate variations in their concentrations to oral health and ecology. An alternative is to focus on variations in the major functions of saliva. An hydroxyapatite-coated microplate model has been developed that simultaneously measures saliva-promoted bacterial viability, bacterial aggregation, and live and dead bacterial adherence, while simulating oral temperature and shearing forces from swallowing. That model was applied to resting whole and stimulated parotid saliva from 149 individuals, using representative strains of Streptococcus crista, S. mutans, and Actinobacillus actinomycetemcomitans. Two major factors were defined by multivariate analysis (this was successful only for whole-saliva). One factor was correlated with aggregation, live adherence and dead adherence for all three strains; the other was correlated with total viability of all three strains. Participants were grouped <25th percentile and >75th percentile for each factor. Those groups were compared for clinical indices of oral health. Caries scores were significantly lower in those with high scores for aggregation-adherence, regardless of whether total viability scores were low or high. Live bacteria always predominated on surfaces when live and dead adherence scores were expressed as ratios. However, participants with high scores for aggregation-adherence showed significantly more dead adherent bacteria than those with low scores (these ratios were uncorrelated with total viability). This finding may indicate that extreme differences in the ability to kill bacteria on surfaces can influence caries risk.
|Original language||English (US)|
|Number of pages||13|
|Journal||Archives of Oral Biology|
|State||Published - 2002|
Bibliographical noteFunding Information:
This work was supported by Public Health Service grant 2 R01 DE 07233 from the National Institute for Dental and Craniofacial Research. Dr Massimo Costalonga from the Minnesota Oral Health Clinical Research Center Clinical Core served as the clinical examiner. We also thank Dr James Hodges of the Minnesota Oral Health Clinical Research Center Biostatistics Core for statistical advice. Facilities provided by the Minnesota Oral Health Clinical Research Center were supported by Public Health Service grant P30 DE09737 from the National Institute for Dental and Craniofacial Research.
- Actinobacillus actinomycetemcomitans
- Dental caries
- Dental plaque
- Periodontal disease
- Streptococcus crista
- Streptococcus mutans