Simultaneous measurement of glucose blood-brain transport constants and metabolic rate in rat brain using in-vivo 1 H MRS

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Cerebral glucose consumption and glucose transport across the blood-brain barrier are crucial to brain function since glucose is the major energy fuel for supporting intense electrophysiological activity associated with neuronal firing and signaling. Therefore, the development of noninvasive methods to measure the cerebral metabolic rate of glucose (CMR glc) and glucose transport constants (K T: half-saturation constant; T max: maximum transport rate) are of importance for understanding glucose transport mechanism and neuroenergetics under various physiological and pathological conditions. In this study, a novel approach able to simultaneously measure CMR glc, K, T and T max via monitoring the dynamic glucose concentration changes in the brain tissue using in-vivo 1 H magnetic resonance spectroscopy (MRS) and in plasma after a brief glucose infusion was proposed and tested using an animal model. The values of CMR glc, T max, and K T were determined to be 0.44±0.17 μmol/g per minute, 1.35±0.47 mol/g per minute, and 13.4±6.8 mmol/L in the rat brain anesthetized with 2% isoflurane. The Monte-Carlo simulations suggest that the measurements of CMR glc and T max are more reliable than that of K T. The overall results indicate that the new approach is robust and reliable for in-vivo measurements of both brain glucose metabolic rate and transport constants, and has potential for human application.

Original languageEnglish (US)
Pages (from-to)1778-1787
Number of pages10
JournalJournal of Cerebral Blood Flow and Metabolism
Volume32
Issue number9
DOIs
StatePublished - Sep 1 2012

Fingerprint

Blood Glucose
Magnetic Resonance Spectroscopy
Glucose
Brain
Isoflurane
Theophylline
Blood-Brain Barrier
Animal Models

Keywords

  • Michaelis-Menten model
  • blood-brain barrier (BBB)
  • blood-brain glucose transport
  • brain glucose metabolism
  • cerebral metabolic rate of glucose (CMRglc)
  • in-vivo 1H MRS

Cite this

@article{839431c140e8465ab91ba9652eb07623,
title = "Simultaneous measurement of glucose blood-brain transport constants and metabolic rate in rat brain using in-vivo 1 H MRS",
abstract = "Cerebral glucose consumption and glucose transport across the blood-brain barrier are crucial to brain function since glucose is the major energy fuel for supporting intense electrophysiological activity associated with neuronal firing and signaling. Therefore, the development of noninvasive methods to measure the cerebral metabolic rate of glucose (CMR glc) and glucose transport constants (K T: half-saturation constant; T max: maximum transport rate) are of importance for understanding glucose transport mechanism and neuroenergetics under various physiological and pathological conditions. In this study, a novel approach able to simultaneously measure CMR glc, K, T and T max via monitoring the dynamic glucose concentration changes in the brain tissue using in-vivo 1 H magnetic resonance spectroscopy (MRS) and in plasma after a brief glucose infusion was proposed and tested using an animal model. The values of CMR glc, T max, and K T were determined to be 0.44±0.17 μmol/g per minute, 1.35±0.47 mol/g per minute, and 13.4±6.8 mmol/L in the rat brain anesthetized with 2{\%} isoflurane. The Monte-Carlo simulations suggest that the measurements of CMR glc and T max are more reliable than that of K T. The overall results indicate that the new approach is robust and reliable for in-vivo measurements of both brain glucose metabolic rate and transport constants, and has potential for human application.",
keywords = "Michaelis-Menten model, blood-brain barrier (BBB), blood-brain glucose transport, brain glucose metabolism, cerebral metabolic rate of glucose (CMRglc), in-vivo 1H MRS",
author = "Fei Du and Yi Zhang and Zhu, {Xiao Hong} and Wei Chen",
year = "2012",
month = "9",
day = "1",
doi = "10.1038/jcbfm.2012.82",
language = "English (US)",
volume = "32",
pages = "1778--1787",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - Simultaneous measurement of glucose blood-brain transport constants and metabolic rate in rat brain using in-vivo 1 H MRS

AU - Du, Fei

AU - Zhang, Yi

AU - Zhu, Xiao Hong

AU - Chen, Wei

PY - 2012/9/1

Y1 - 2012/9/1

N2 - Cerebral glucose consumption and glucose transport across the blood-brain barrier are crucial to brain function since glucose is the major energy fuel for supporting intense electrophysiological activity associated with neuronal firing and signaling. Therefore, the development of noninvasive methods to measure the cerebral metabolic rate of glucose (CMR glc) and glucose transport constants (K T: half-saturation constant; T max: maximum transport rate) are of importance for understanding glucose transport mechanism and neuroenergetics under various physiological and pathological conditions. In this study, a novel approach able to simultaneously measure CMR glc, K, T and T max via monitoring the dynamic glucose concentration changes in the brain tissue using in-vivo 1 H magnetic resonance spectroscopy (MRS) and in plasma after a brief glucose infusion was proposed and tested using an animal model. The values of CMR glc, T max, and K T were determined to be 0.44±0.17 μmol/g per minute, 1.35±0.47 mol/g per minute, and 13.4±6.8 mmol/L in the rat brain anesthetized with 2% isoflurane. The Monte-Carlo simulations suggest that the measurements of CMR glc and T max are more reliable than that of K T. The overall results indicate that the new approach is robust and reliable for in-vivo measurements of both brain glucose metabolic rate and transport constants, and has potential for human application.

AB - Cerebral glucose consumption and glucose transport across the blood-brain barrier are crucial to brain function since glucose is the major energy fuel for supporting intense electrophysiological activity associated with neuronal firing and signaling. Therefore, the development of noninvasive methods to measure the cerebral metabolic rate of glucose (CMR glc) and glucose transport constants (K T: half-saturation constant; T max: maximum transport rate) are of importance for understanding glucose transport mechanism and neuroenergetics under various physiological and pathological conditions. In this study, a novel approach able to simultaneously measure CMR glc, K, T and T max via monitoring the dynamic glucose concentration changes in the brain tissue using in-vivo 1 H magnetic resonance spectroscopy (MRS) and in plasma after a brief glucose infusion was proposed and tested using an animal model. The values of CMR glc, T max, and K T were determined to be 0.44±0.17 μmol/g per minute, 1.35±0.47 mol/g per minute, and 13.4±6.8 mmol/L in the rat brain anesthetized with 2% isoflurane. The Monte-Carlo simulations suggest that the measurements of CMR glc and T max are more reliable than that of K T. The overall results indicate that the new approach is robust and reliable for in-vivo measurements of both brain glucose metabolic rate and transport constants, and has potential for human application.

KW - Michaelis-Menten model

KW - blood-brain barrier (BBB)

KW - blood-brain glucose transport

KW - brain glucose metabolism

KW - cerebral metabolic rate of glucose (CMRglc)

KW - in-vivo 1H MRS

UR - http://www.scopus.com/inward/record.url?scp=84865991408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865991408&partnerID=8YFLogxK

U2 - 10.1038/jcbfm.2012.82

DO - 10.1038/jcbfm.2012.82

M3 - Article

C2 - 22714049

AN - SCOPUS:84865991408

VL - 32

SP - 1778

EP - 1787

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 9

ER -