Simultaneous laser-induced fluorescence and scattering detection of individual particles separated by capillary electrophoresis

Dmitry Andreyev, Edgar A. Arriaga

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

This technical note describes a detector capable of simultaneously monitoring scattering and fluorescence signals of individual particles separated by capillary electrophoresis. Due to its nonselective nature, scattering alone is not sufficient to identify analyte particles. However, when the analyte particles are fluorescent, the detector described here is able to identify simultaneously occurring scattering and fluorescent signals, even when contaminating particles (i.e., nonfluorescent) are present. Both fluorescent polystyrene particles and 10-nonyl acridine orange (NAO)-labeled mitochondria were used as models. Fluorescence versus scattering (FVS) plots made it possible to identify two types of particles and a contaminant in a mixture of polystyrene particles. We also analyzed NAO-labeled mitochondria before and after cryogenic storage; the mitochondria FVS plots changed with storage, which suggests that the detector reported here is suitable for monitoring subtle changes in mitochondrial morphology that would not be revealed by monitoring only fluorescence or scattering signals.

Original languageEnglish (US)
Pages (from-to)5474-5478
Number of pages5
JournalAnalytical chemistry
Volume79
Issue number14
DOIs
StatePublished - Jul 15 2007

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