TY - JOUR
T1 - Simultaneous in situ detection of viral RNA and antigens
AU - Brahic, M.
AU - Haase, A. T.
AU - Cash, E.
PY - 1984
Y1 - 1984
N2 - We have combined in situ hybridization and immunocytochemistry to detect RNA and proteins in the same cell. We envision a wide range of applications for this method, and, as one example, we show that viral genomes and capsid antigens can be detected simultaneously in paraffin sections of the central nervous system of mice infected with Theiler's virus, the causative agent of a slow demyelinating disease.
AB - We have combined in situ hybridization and immunocytochemistry to detect RNA and proteins in the same cell. We envision a wide range of applications for this method, and, as one example, we show that viral genomes and capsid antigens can be detected simultaneously in paraffin sections of the central nervous system of mice infected with Theiler's virus, the causative agent of a slow demyelinating disease.
UR - http://www.scopus.com/inward/record.url?scp=0347074049&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347074049&partnerID=8YFLogxK
U2 - 10.1073/pnas.81.17.5445
DO - 10.1073/pnas.81.17.5445
M3 - Article
C2 - 6206496
AN - SCOPUS:0347074049
SN - 0027-8424
VL - 81
SP - 5445
EP - 5448
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 17 I
ER -