Simultaneous edited MRS of GABA, glutathione, and ethanol

Muhammad G. Saleh; Min Wang; Mark Mikkelsen; Steve C.N. Hui; Georg Oeltzschner; Jeff Boissoneault; Bethany Stennett; Richard A.E. Edden; Eric C. Porges

doi:10.1002/nbm.4227

Simultaneous edited MRS of GABA, glutathione, and ethanol

Muhammad G. Saleh, Min Wang, Mark Mikkelsen, Steve C.N. Hui, Georg Oeltzschner, Jeff Boissoneault, Bethany Stennett, Richard A.E. Edden, Eric C. Porges

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The aim of this work was to develop simultaneous edited MRS of γ-aminobutyric acid (GABA), glutathione (GSH), and ethanol (EtOH) using Hadamard encoding and reconstruction of MEGA-edited spectroscopy (HERMES) at 3T. Density-matrix simulations of HERMES were carried out and compared with phantom experiments. In vivo experiments were performed in six healthy volunteers about 30 min after alcohol consumption. Simulations of HERMES showed GABA-, GSH-, and EtOH-edited spectra with low levels of crosstalk and excellent agreement with phantom spectra. In vivo experiments showed well edited GABA signals at 3.0 ppm, GSH at 2.95 ppm, and EtOH at 1.18 ppm in the respective Hadamard combination spectra. Measured integral ratios were 0.082 ± 0.012 for GABA/Cr, 0.037 ± 0.006 for GSH/Cr, and 0.305 ± 0.129 for EtOH/Cr. Simulated, phantom, and in vivo measurements of HERMES show excellent separation of GABA-, GSH-, and EtOH-edited signals with negligible levels of crosstalk. HERMES allows a threefold acceleration of editing while maintaining spectral quality compared with sequentially acquired MEGA-PRESS measurements.

Original language	English (US)
Article number	e4227
Journal	NMR in biomedicine
Volume	33
Issue number	4
DOIs	https://doi.org/10.1002/nbm.4227
State	Published - Apr 1 2020
Externally published	Yes

Bibliographical note

Funding Information:
We would like to thank Brent Foster, Genelle Samson, and Joy Keyanni for their contributions to data collection. Research reported in this publication was supported by the National Institute on Alcohol Abuse and Alcoholism and the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under award numbers: RAE: R01 EB023963, R01 EB016089, and P41 EB015909; JB and BS: R01AA025337; ECP: K01AA025306. A portion of this work was performed in the McKnight Brain Institute at the National High Magnetic Field Laboratory's AMRIS Facility, which is supported by National Science Foundation Cooperative Agreement No. DMR-1157490* and the State of Florida. This work was supported in part by an NIH award, S10OD021726, for High End Instrumentation.

Funding Information:
We would like to thank Brent Foster, Genelle Samson, and Joy Keyanni for their contributions to data collection. Research reported in this publication was supported by the National Institute on Alcohol Abuse and Alcoholism and the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under award numbers: RAE: R01 EB023963, R01 EB016089, and P41 EB015909; JB and BS: R01AA025337; ECP: K01AA025306. A portion of this work was performed in the McKnight Brain Institute at the National High Magnetic Field Laboratory's AMRIS Facility, which is supported by National Science Foundation Cooperative Agreement No. DMR‐1157490* and the State of Florida. This work was supported in part by an NIH award, S10OD021726, for High End Instrumentation.

Publisher Copyright:
© 2020 John Wiley & Sons, Ltd.

Keywords

edited MRS
ethanol
GABA
GSH
HERMES

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10.1002/nbm.4227

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title = "Simultaneous edited MRS of GABA, glutathione, and ethanol",

abstract = "The aim of this work was to develop simultaneous edited MRS of γ-aminobutyric acid (GABA), glutathione (GSH), and ethanol (EtOH) using Hadamard encoding and reconstruction of MEGA-edited spectroscopy (HERMES) at 3T. Density-matrix simulations of HERMES were carried out and compared with phantom experiments. In vivo experiments were performed in six healthy volunteers about 30 min after alcohol consumption. Simulations of HERMES showed GABA-, GSH-, and EtOH-edited spectra with low levels of crosstalk and excellent agreement with phantom spectra. In vivo experiments showed well edited GABA signals at 3.0 ppm, GSH at 2.95 ppm, and EtOH at 1.18 ppm in the respective Hadamard combination spectra. Measured integral ratios were 0.082 ± 0.012 for GABA/Cr, 0.037 ± 0.006 for GSH/Cr, and 0.305 ± 0.129 for EtOH/Cr. Simulated, phantom, and in vivo measurements of HERMES show excellent separation of GABA-, GSH-, and EtOH-edited signals with negligible levels of crosstalk. HERMES allows a threefold acceleration of editing while maintaining spectral quality compared with sequentially acquired MEGA-PRESS measurements.",

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author = "Saleh, {Muhammad G.} and Min Wang and Mark Mikkelsen and Hui, {Steve C.N.} and Georg Oeltzschner and Jeff Boissoneault and Bethany Stennett and Edden, {Richard A.E.} and Porges, {Eric C.}",

note = "Funding Information: We would like to thank Brent Foster, Genelle Samson, and Joy Keyanni for their contributions to data collection. Research reported in this publication was supported by the National Institute on Alcohol Abuse and Alcoholism and the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under award numbers: RAE: R01 EB023963, R01 EB016089, and P41 EB015909; JB and BS: R01AA025337; ECP: K01AA025306. A portion of this work was performed in the McKnight Brain Institute at the National High Magnetic Field Laboratory's AMRIS Facility, which is supported by National Science Foundation Cooperative Agreement No. DMR-1157490* and the State of Florida. This work was supported in part by an NIH award, S10OD021726, for High End Instrumentation. Funding Information: We would like to thank Brent Foster, Genelle Samson, and Joy Keyanni for their contributions to data collection. Research reported in this publication was supported by the National Institute on Alcohol Abuse and Alcoholism and the National Institute of Biomedical Imaging and Bioengineering of the National Institutes of Health under award numbers: RAE: R01 EB023963, R01 EB016089, and P41 EB015909; JB and BS: R01AA025337; ECP: K01AA025306. A portion of this work was performed in the McKnight Brain Institute at the National High Magnetic Field Laboratory's AMRIS Facility, which is supported by National Science Foundation Cooperative Agreement No. DMR‐1157490* and the State of Florida. This work was supported in part by an NIH award, S10OD021726, for High End Instrumentation. Publisher Copyright: {\textcopyright} 2020 John Wiley & Sons, Ltd.",

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AU - Oeltzschner, Georg

AU - Boissoneault, Jeff

AU - Stennett, Bethany

AU - Edden, Richard A.E.

AU - Porges, Eric C.

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Simultaneous edited MRS of GABA, glutathione, and ethanol

Abstract

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