Simultaneous determination of ginkgolides A, B, C and bilobalide in plasma by LC-MS/MS and its application to the pharmacokinetic study of Ginkgo biloba extract in rats

Jiashu Xie, Cungang Ding, Qinghua Ge, Zhen Zhou, Xiaojin Zhi

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

A new liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of ginkgolides (includes ginkgolide C for the first time) and bilobalide in plasma is presented. Ketoprofen was used as an internal standard, and sample pre-treatment consisted of a liquid-liquid extraction. Chromatographic separation was achieved on a 5 μm Shiseido C8 column (150 mm × 2.0 mm i.d., particle size 5 μm) with a mobile phase consisting of methanol/6 mM ammonium acetate (60/40, v/v) at a flow rate of 0.3 ml/min. A tandem mass spectrometric detection was conducted using multiple reaction monitoring (MRM) under negative ionization mode with an atmospheric pressure chemical ionization (APCI) interface. The method was validated in terms of intra- and inter-day precision (<12.7%), accuracy (within ±7.0%), linearity, specificity and stability. In addition, matrix effects of ginkgolides and bilobalide in plasma were evaluated in different reconstitution solvents. Smaller matrix effects were observed for reconstitution solvents containing less organic solvent. The method has been successfully applied to a pharmacokinetic study of Ginkgo biloba extract in rats after intravenous administration. This is the first report of pharmacokinetic data for ginkgolide C.

Original languageEnglish (US)
Pages (from-to)87-94
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume864
Issue number1-2
DOIs
StatePublished - Mar 15 2008
Externally publishedYes

Keywords

  • Bilobalide
  • Ginkgolides
  • LC-MS/MS
  • Matrix effect
  • Pharmacokinetic
  • Simultaneous analysis

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