Etretinate is a synthetic aromatic retinoid used in the treatment of psoriasis and other disorders affecting the skin. Acitretin is the primary active metabolite of etretinate. The in situ perfused rat liver model was used to study the first-pass hepatic metabolism of etretinate and acitretin and a reliable method of quantifying etretinate and its metabolites was needed. Previously published assays allow for the simultaneous quantitation of etretinate and acitretin in blood or plasma. This paper describes an accurate and reliable reversed-phase HPLC method for the determination of etretinate, acitretin and their metabolites in whole perfusate, plasma, bile and hepatic tissue.
|Original language||English (US)|
|Number of pages||9|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|State||Published - May 5 1995|
Bibliographical noteFunding Information:
This work was supported by Hoffmann-La Roche Inc. and the American Foundation for PharmaceuticaEl ducationand was submitteda s part of the M.S. thesisr equirementfso r Mark A. Decker at the University of Minnesota in January 1994.