Simultaneous assessment of cerebral glucose and oxygen metabolism and perfusion in rats using interleaved deuterium (2H) and oxygen-17 (17O) MRS

Guangle Zhang, Parker Jenkins, Wei Zhu, Wei Chen, Xiao Hong Zhu

Research output: Contribution to journalArticlepeer-review

Abstract

Cerebral glucose and oxygen metabolism and blood perfusion play key roles in neuroenergetics and oxidative phosphorylation to produce adenosine triphosphate (ATP) energy molecules in supporting cellular activity and brain function. Their impairments have been linked to numerous brain disorders. This study aimed to develop an in vivo magnetic resonance spectroscopy (MRS) method capable of simultaneously assessing and quantifying the major cerebral metabolic rates of glucose (CMRGlc) and oxygen (CMRO2) consumption, lactate formation (CMRLac), and tricarboxylic acid (TCA) cycle (VTCA); cerebral blood flow (CBF); and oxygen extraction fraction (OEF) via a single dynamic MRS measurement using an interleaved deuterium (2H) and oxygen-17 (17O) MRS approach. We introduced a single-loop multifrequency radio-frequency (RF) surface coil that can be used to acquire proton (1H) magnetic resonance imaging (MRI) or interleaved low-γ X-nuclei 2H and 17O MRS. By combining this RF coil with a modified MRS pulse sequence, 17O-isotope-labeled oxygen gas inhalation, and intravenous 2H-isotope-labeled glucose administration, we demonstrate for the first time the feasibility of simultaneously and quantitatively measuring six important physiological parameters, CMRGlc, CMRO2, CMRLac, VTCA, CBF, and OEF, in rat brains at 16.4 T. The interleaved 2H–17O MRS technique should be readily adapted to image and study cerebral energy metabolism and perfusion in healthy and diseased brains.

Original languageEnglish (US)
Article numbere5284
JournalNMR in biomedicine
Volume38
Issue number1
DOIs
StatePublished - Jan 2025

Bibliographical note

Publisher Copyright:
© 2024 The Author(s). NMR in Biomedicine published by John Wiley & Sons Ltd.

Keywords

  • brain energy metabolism and perfusion
  • interleaved H–O MRS technique
  • multifrequency RF surface coil
  • ultrahigh field
  • X-nuclear MRS and imaging

PubMed: MeSH publication types

  • Journal Article

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