Simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, N′-nitrosonornicotine, and cotinine by liquid chromatography-tandem mass-spectrometry

Sampada S. Nikam; Murari Gurjar; Hitesh Singhavi; Anand Patil; Arjun Singh; Peter Villalta; Pankaj Chaturvedi; Samir S. Khariwala; Vikram Gota; Irina Stepanov

doi:10.1038/s41598-021-99259-z

Simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, N′-nitrosonornicotine, and cotinine by liquid chromatography-tandem mass-spectrometry

Sampada S. Nikam, Murari Gurjar, Hitesh Singhavi, Anand Patil, Arjun Singh, Peter Villalta, Pankaj Chaturvedi, Samir S. Khariwala, Vikram Gota, Irina Stepanov

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Biomarkers of exposure to harmful tobacco constituents are key tools for identifying individuals at risk and developing interventions and tobacco control measures. However, tobacco biomarker studies are scarce in many parts of the world with high prevalence of tobacco use. Our goal was to establish a robust method for simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), N′-nitrosonornicotine (NNN), and cotinine at the Advanced Centre for Treatment, Research and Education in Cancer (ACTREC) in Mumbai, India. These biomarkers are validated measures of exposure to the carcinogenic tobacco nitrosamines 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and NNN and the addictive alkaloid nicotine, respectively. The established method is characterized by excellent accuracy, linearity, and precision, and was successfully applied to the analysis of 15 smokeless tobacco (SLT) users and 15 non-users of tobacco recruited in Mumbai. This is the first report of establishment of such procedure in a laboratory in India, which offers the first in-country capacity for research on tobacco carcinogenesis in Indian SLT users.

Original language	English (US)
Article number	20007
Journal	Scientific reports
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41598-021-99259-z
State	Published - Dec 2021

Bibliographical note

Funding Information:
The work reported here was supported by funds from the University of Minnesota’s Center for Global Health and Social Responsibility (Khariwala), FIC/NIH Grant R01-TW010651 (Khariwala/Stepanov/Chaturvedi, MPIs), and in part by R01-CA180880 (Stepanov). LC-MS/MS analysis was supported in part by XII Plan project funding from the Department of Atomic Energy, Government of India. The content is solely the responsibility of the authors and does not represent the official views of the funding agencies.

Publisher Copyright:
© 2021, The Author(s).

PubMed: MeSH publication types

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Publisher link

10.1038/s41598-021-99259-z

Find It

Find It at U of M Libraries

Cite this

Nikam, S. S., Gurjar, M., Singhavi, H., Patil, A., Singh, A., Villalta, P., Chaturvedi, P., Khariwala, S. S., Gota, V., & Stepanov, I. (2021). Simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, N′-nitrosonornicotine, and cotinine by liquid chromatography-tandem mass-spectrometry. Scientific reports, 11(1), Article 20007. https://doi.org/10.1038/s41598-021-99259-z

Nikam, SS, Gurjar, M, Singhavi, H, Patil, A, Singh, A, Villalta, P, Chaturvedi, P, Khariwala, SS, Gota, V & Stepanov, I 2021, 'Simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, N′-nitrosonornicotine, and cotinine by liquid chromatography-tandem mass-spectrometry', Scientific reports, vol. 11, no. 1, 20007. https://doi.org/10.1038/s41598-021-99259-z

@article{5c272ea7e82b4366864329af2b20deac,

title = "Simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, N′-nitrosonornicotine, and cotinine by liquid chromatography-tandem mass-spectrometry",

abstract = "Biomarkers of exposure to harmful tobacco constituents are key tools for identifying individuals at risk and developing interventions and tobacco control measures. However, tobacco biomarker studies are scarce in many parts of the world with high prevalence of tobacco use. Our goal was to establish a robust method for simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), N′-nitrosonornicotine (NNN), and cotinine at the Advanced Centre for Treatment, Research and Education in Cancer (ACTREC) in Mumbai, India. These biomarkers are validated measures of exposure to the carcinogenic tobacco nitrosamines 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and NNN and the addictive alkaloid nicotine, respectively. The established method is characterized by excellent accuracy, linearity, and precision, and was successfully applied to the analysis of 15 smokeless tobacco (SLT) users and 15 non-users of tobacco recruited in Mumbai. This is the first report of establishment of such procedure in a laboratory in India, which offers the first in-country capacity for research on tobacco carcinogenesis in Indian SLT users.",

author = "Nikam, {Sampada S.} and Murari Gurjar and Hitesh Singhavi and Anand Patil and Arjun Singh and Peter Villalta and Pankaj Chaturvedi and Khariwala, {Samir S.} and Vikram Gota and Irina Stepanov",

note = "Funding Information: The work reported here was supported by funds from the University of Minnesota{\textquoteright}s Center for Global Health and Social Responsibility (Khariwala), FIC/NIH Grant R01-TW010651 (Khariwala/Stepanov/Chaturvedi, MPIs), and in part by R01-CA180880 (Stepanov). LC-MS/MS analysis was supported in part by XII Plan project funding from the Department of Atomic Energy, Government of India. The content is solely the responsibility of the authors and does not represent the official views of the funding agencies. Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = dec,

doi = "10.1038/s41598-021-99259-z",

language = "English (US)",

volume = "11",

journal = "Scientific reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - Simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, N′-nitrosonornicotine, and cotinine by liquid chromatography-tandem mass-spectrometry

AU - Nikam, Sampada S.

AU - Gurjar, Murari

AU - Singhavi, Hitesh

AU - Patil, Anand

AU - Singh, Arjun

AU - Villalta, Peter

AU - Chaturvedi, Pankaj

AU - Khariwala, Samir S.

AU - Gota, Vikram

AU - Stepanov, Irina

N1 - Funding Information: The work reported here was supported by funds from the University of Minnesota’s Center for Global Health and Social Responsibility (Khariwala), FIC/NIH Grant R01-TW010651 (Khariwala/Stepanov/Chaturvedi, MPIs), and in part by R01-CA180880 (Stepanov). LC-MS/MS analysis was supported in part by XII Plan project funding from the Department of Atomic Energy, Government of India. The content is solely the responsibility of the authors and does not represent the official views of the funding agencies. Publisher Copyright: © 2021, The Author(s).

PY - 2021/12

Y1 - 2021/12

N2 - Biomarkers of exposure to harmful tobacco constituents are key tools for identifying individuals at risk and developing interventions and tobacco control measures. However, tobacco biomarker studies are scarce in many parts of the world with high prevalence of tobacco use. Our goal was to establish a robust method for simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), N′-nitrosonornicotine (NNN), and cotinine at the Advanced Centre for Treatment, Research and Education in Cancer (ACTREC) in Mumbai, India. These biomarkers are validated measures of exposure to the carcinogenic tobacco nitrosamines 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and NNN and the addictive alkaloid nicotine, respectively. The established method is characterized by excellent accuracy, linearity, and precision, and was successfully applied to the analysis of 15 smokeless tobacco (SLT) users and 15 non-users of tobacco recruited in Mumbai. This is the first report of establishment of such procedure in a laboratory in India, which offers the first in-country capacity for research on tobacco carcinogenesis in Indian SLT users.

AB - Biomarkers of exposure to harmful tobacco constituents are key tools for identifying individuals at risk and developing interventions and tobacco control measures. However, tobacco biomarker studies are scarce in many parts of the world with high prevalence of tobacco use. Our goal was to establish a robust method for simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), N′-nitrosonornicotine (NNN), and cotinine at the Advanced Centre for Treatment, Research and Education in Cancer (ACTREC) in Mumbai, India. These biomarkers are validated measures of exposure to the carcinogenic tobacco nitrosamines 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and NNN and the addictive alkaloid nicotine, respectively. The established method is characterized by excellent accuracy, linearity, and precision, and was successfully applied to the analysis of 15 smokeless tobacco (SLT) users and 15 non-users of tobacco recruited in Mumbai. This is the first report of establishment of such procedure in a laboratory in India, which offers the first in-country capacity for research on tobacco carcinogenesis in Indian SLT users.

UR - http://www.scopus.com/inward/record.url?scp=85116762411&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85116762411&partnerID=8YFLogxK

U2 - 10.1038/s41598-021-99259-z

DO - 10.1038/s41598-021-99259-z

M3 - Article

C2 - 34625573

AN - SCOPUS:85116762411

SN - 2045-2322

VL - 11

JO - Scientific reports

JF - Scientific reports

IS - 1

M1 - 20007

ER -

Simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, N′-nitrosonornicotine, and cotinine by liquid chromatography-tandem mass-spectrometry

Abstract

Bibliographical note

PubMed: MeSH publication types

UN SDGs

Publisher link

Find It

Other files and links

Fingerprint

Cite this