Simultaneous analysis of furosemide and bumetanide in horse plasma using high performance liquid chromatography

Ashok K Singh, Camille McArdle, Brad Gordon, M. Ashraf, K. Granley

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A high performance liquid chromatographic method is described for the simultaneous determination of furosemide and bumetanide in horse plasma. The C8 (3 μm) reversed phase column (4.8 × 150 mm) provided clear separation of furosemide and bumetanide with other components present in the horse plasma. The detection limit for both the drugs was 10 ng/mL. Both drugs were stable in plasma (at natural or acidic pH) for up to 24 h. The method is sufficiently sensitive to detect furosemide levels in plasma obtained from horses receiving a therapeutic dose of furosemide.

Original languageEnglish (US)
Pages (from-to)262-265
Number of pages4
JournalBiomedical Chromatography
Volume3
Issue number6
DOIs
StatePublished - Nov 1989

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