Simultaneous analysis of flunixin, naproxen, ethacrynic acid, indomethacin, phenylbutazone, mefenamic acid and thiosalicycic acid in plasma and urine by high-performance liquid chromatography and gas chromatography-mass spectrometry

Ashok K. Singh, Yin Jang, Usha Mishra, Kae Granley

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

Simple and reproducible high-performance liquid chromatographic (HPLC) and gas chromatographic-mass spectrometric (GC-MS) methods have been developed for the simultaneous analysis of several acidic drugs in horse plasma and urine. Although the capillary GC-MS column provided better separation of the drugs than the reversed-phase C8 (3 μm, 75 mm) HPLC column, the total analysis time with HPLC was shorter than the total analysis time with GC-MS. The HPLC system equipped with a diode-array detector provided simultaneous screening (limit of detection 100-500 ng/ml) and confirmation (limit 1.0 μg/ml) of the drugs. The HPLC system equipped with fixed-wavelength ultraviolet and fluorescence detectors provided a relatively sensitive screening [limit of detection 50-150 ng/ml for ultraviolet and 10 ng/ml for fluorescence (naproxen only) detectors] of the drugs. However, the positive samples had to be confirmed by using either the diode-array detector or the GC-MS system. The GC-MS system provided simultaneous screening and confirmation of the drugs at very low concentrations (20-50 ng/ml).

Original languageEnglish (US)
Pages (from-to)351-361
Number of pages11
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume568
Issue number2
DOIs
StatePublished - Aug 23 1991

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