Polarization optimized experiments (POE) utilize orphan spin operators for the acquisition of multiple experiments in solid-state NMR spectroscopy. The initial applications of these experiments was limited to direct detection experiments. In this Annual Report, we summarize our progress to include 1H-detected (inverse detection) POE. We describe the different strategies to exploit orphan spin polarization to generate multiple nD spectra. To generalize our experiments, we show the application to standard microcrystalline samples of GB1 protein and more challenging membrane protein sample reconstituted in lipid membranes. These new 1H-detected POE can be used either to enhance signal intensity or collect several datasets simultaneously with significant time-saving.
|Original language||English (US)|
|Title of host publication||Annual Reports on NMR Spectroscopy|
|Editors||Graham A. Webb|
|Publisher||Academic Press Inc.|
|Number of pages||22|
|State||Published - Jan 2021|
|Name||Annual Reports on NMR Spectroscopy|
Bibliographical noteFunding Information:
The experiments were carried out at Minnesota NMR Center with the support of the National Institutes of Health (GM 64742, HL 144130, and 1S10OD021536).
© 2021 Elsevier Ltd
- fast MAS
- Multi-receiver acquisitions
- Orphan spin polarization
- Solid-state NMR