Simultaneous acquisition of multiple fast MAS solid-state NMR experiments via orphan spin polarization

Tata Gopinath, Gianluigi Veglia

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Polarization optimized experiments (POE) utilize orphan spin operators for the acquisition of multiple experiments in solid-state NMR spectroscopy. The initial applications of these experiments was limited to direct detection experiments. In this Annual Report, we summarize our progress to include 1H-detected (inverse detection) POE. We describe the different strategies to exploit orphan spin polarization to generate multiple nD spectra. To generalize our experiments, we show the application to standard microcrystalline samples of GB1 protein and more challenging membrane protein sample reconstituted in lipid membranes. These new 1H-detected POE can be used either to enhance signal intensity or collect several datasets simultaneously with significant time-saving.

Original languageEnglish (US)
Title of host publicationAnnual Reports on NMR Spectroscopy
EditorsGraham A. Webb
PublisherAcademic Press Inc.
Pages247-268
Number of pages22
ISBN (Print)9780128246092
DOIs
StatePublished - Jan 2021

Publication series

NameAnnual Reports on NMR Spectroscopy
Volume102
ISSN (Print)0066-4103

Bibliographical note

Funding Information:
The experiments were carried out at Minnesota NMR Center with the support of the National Institutes of Health (GM 64742, HL 144130, and 1S10OD021536).

Publisher Copyright:
© 2021 Elsevier Ltd

Keywords

  • DUMAS
  • fast MAS
  • MAESTOSO
  • MEIOSIS
  • Multi-receiver acquisitions
  • Orphan spin polarization
  • POE
  • SIM-CP
  • Solid-state NMR

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