Simulated peripheral nerve regeneration in magnetically aligned collagen gels derivatized with laminin peptides and seeded with Schwann cells

Benjamin I. Rosner, Naren Dubey, Paul C. Letourneau, Robert T. Tranquillo

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

High strength magnetic fields were used to align collagen gel formed into 4 mm diameter rods during the self assembly of type I collagen monomers into fibrils. We developed an in vitro assay to study neurite elongation into magnetically aligned collagen gel rods (CGRs) from rat dorsal root ganglia (DRG) placed onto one end of the rods. Enhancement of invasion due to growth cone contact guidance in these in vitro experiments has been manifested as improved regeneration in an in vivo study in mice. Preliminary experimental results using pre-seeded Schwann cells and collagen gels with derivatized laminin peptides to stimulate as well as direct neurite elongation in our CGR-DRG invasion assay are presented. These results may translate into an improved method of entubulation repair of transected peripheral nerves by directing as well as stimulating neurite growth through a tube filled with magnetically aligned collagen gel.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Number of pages1
ISBN (Print)0780356756
StatePublished - Dec 1 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
ISSN (Print)0589-1019

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

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