SIMPLE TEST FOR CHARACTERIZING SILANOPHILIC INTERACTIONS OF REVERSED-PHASE COLUMNS BASED ON THE CHROMATOGRAPHIC BEHAVIOR OF CYCLIC TETRAAZA COMPOUNDS.

Paul C. Sadek, Peter W. Carr

Research output: Contribution to journalArticlepeer-review

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Abstract

The silanophilic character of 16 reversed-phase high performance liquid chromatographic columns is evaluated with dimethyldiphenylcyclam, a cyclic tetraaza macrocycle. The method is rapid, does not require the removal of packing material, and uses a water-miscible solvent. The results demonstrate two points: first, cyclic tetraaza macrocycles offer substantial benefits over currently used silanophilic agents; second, the method can easily differentiate the performance of various columns in terms of their relative hydrophobic and silanophilic contributions to solute retention.

Original languageEnglish (US)
Pages (from-to)314-320
Number of pages7
JournalJournal of Chromatographic Science
Volume21
Issue number7
DOIs
StatePublished - Jan 1 1983

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