Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics

Dominik Kopczynski; Andreas Hentschel; Cristina Coman; Nils Helge Schebb; Thorsten Hornemann; Douglas G. Mashek; Nicole M. Hartung; Olga Shevchuk; Hans Frieder Schött; Kristina Lorenz; Federico Torta; Bo Burla; René P. Zahedi; Albert Sickmann; Michael R. Kreutz; Christer S. Ejsing; Jan Medenbach; Robert Ahrends

doi:10.1021/acs.analchem.0c02793

Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics

Dominik Kopczynski, Andreas Hentschel, Cristina Coman, Nils Helge Schebb, Thorsten Hornemann, Douglas G. Mashek, Nicole M. Hartung, Olga Shevchuk, Hans Frieder Schött, Kristina Lorenz, Federico Torta, Bo Burla, René P. Zahedi, Albert Sickmann, Michael R. Kreutz, Christer S. Ejsing, Jan Medenbach, Robert Ahrends

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1 Scopus citations

Abstract

We introduce STAMPS, a pathway-centric web service for the development of targeted proteomics assays. STAMPS guides the user by providing several intuitive interfaces for a rapid and simplified method design. Applying our curated framework to signaling and metabolic pathways, we reduced the average assay development time by a factor of ∼150 and revealed that the insulin signaling is actively controlled by protein abundance changes in insulin-sensitive and -resistance states. Although at the current state STAMPS primarily contains mouse data, it was designed for easy extension with additional organisms.

Original language	English (US)
Pages (from-to)	13672-13676
Number of pages	5
Journal	Analytical Chemistry
Volume	92
Issue number	20
DOIs	https://doi.org/10.1021/acs.analchem.0c02793
State	Published - Oct 20 2020

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© 2020 American Chemical Society.

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Research Support, Non-U.S. Gov't

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Kopczynski, D., Hentschel, A., Coman, C., Schebb, N. H., Hornemann, T., Mashek, D. G., Hartung, N. M., Shevchuk, O., Schött, H. F., Lorenz, K., Torta, F., Burla, B., Zahedi, R. P., Sickmann, A., Kreutz, M. R., Ejsing, C. S., Medenbach, J., & Ahrends, R. (2020). Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics. Analytical Chemistry, 92(20), 13672-13676. https://doi.org/10.1021/acs.analchem.0c02793

Kopczynski, D, Hentschel, A, Coman, C, Schebb, NH, Hornemann, T, Mashek, DG, Hartung, NM, Shevchuk, O, Schött, HF, Lorenz, K, Torta, F, Burla, B, Zahedi, RP, Sickmann, A, Kreutz, MR, Ejsing, CS, Medenbach, J & Ahrends, R 2020, 'Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics', Analytical Chemistry, vol. 92, no. 20, pp. 13672-13676. https://doi.org/10.1021/acs.analchem.0c02793

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AU - Coman, Cristina

AU - Schebb, Nils Helge

AU - Hornemann, Thorsten

AU - Mashek, Douglas G.

AU - Hartung, Nicole M.

AU - Shevchuk, Olga

AU - Schött, Hans Frieder

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AU - Torta, Federico

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AU - Zahedi, René P.

AU - Sickmann, Albert

AU - Kreutz, Michael R.

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AU - Medenbach, Jan

AU - Ahrends, Robert

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Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics

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