Simple Targeted Assays for Metabolic Pathways and Signaling: A Powerful Tool for Targeted Proteomics

Dominik Kopczynski, Andreas Hentschel, Cristina Coman, Nils Helge Schebb, Thorsten Hornemann, Douglas G. Mashek, Nicole M. Hartung, Olga Shevchuk, Hans Frieder Schött, Kristina Lorenz, Federico Torta, Bo Burla, René P. Zahedi, Albert Sickmann, Michael R. Kreutz, Christer S. Ejsing, Jan Medenbach, Robert Ahrends

Research output: Contribution to journalArticlepeer-review

Abstract

We introduce STAMPS, a pathway-centric web service for the development of targeted proteomics assays. STAMPS guides the user by providing several intuitive interfaces for a rapid and simplified method design. Applying our curated framework to signaling and metabolic pathways, we reduced the average assay development time by a factor of ∼150 and revealed that the insulin signaling is actively controlled by protein abundance changes in insulin-sensitive and -resistance states. Although at the current state STAMPS primarily contains mouse data, it was designed for easy extension with additional organisms.

Original languageEnglish (US)
Pages (from-to)13672-13676
Number of pages5
JournalAnalytical Chemistry
Volume92
Issue number20
DOIs
StatePublished - Oct 20 2020

Bibliographical note

Funding Information:
This study was supported by the Ministerium für Innovation, Wissenschaft und Forschung des Landes Nordrhein-Westfalen, the Senatsverwaltung für Wirtschaft, Technologie und Forschung des Landes Berlin, the Bundesministerium für Bildung und Forschung and by the BMBF de.NBI program (Code 031L0108A to D.K., R.A., and A.S.). Furthermore, this work was supported by the Danish Council for Independent Research|Natural Sciences (DFF-6108-00493 to C.S.E.), the Lundbeckfonden (R54-A5858 to C.S.E.), the VILLUM Foundation (VKR023439 to C.S.E.), and the VILLUM Center for Bioanalytical Sciences (VKR023179 to C.S.E.). The work was further supported by Leibniz Foundation Leibniz Pakt für Forschung SAW-2017-ISAS-2 and SynMetAge.

Publisher Copyright:
© 2020 American Chemical Society.

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't

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