Simple methods of rabbit hemolytic complement component assays

J. F. Lindorfer; J. S.C. Fong; R. A. Good

doi:10.1159/000231156

Simple methods of rabbit hemolytic complement component assays

J. F. Lindorfer, J. S.C. Fong, R. A. Good

Biomedical Sciences

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Abstract

Simple methods for the titration of rabbit C1 and C5-9 have been developed which do not require purified complement components. The stable cellular intermediate, sheep erythrocyte - rabbit antibody - bovine complement C4 and C2 (SHEACBo42) is used for these assay methods with kinetic studies on the interaction between rabbit C1 and C5-9 with SHEACBo42 forming the basis for the methods. Furthermore, these assay methods have many applications for the evaluation of in vivo complement activation of both the classical and alternate pathways.

Original language	English (US)
Pages (from-to)	539-551
Number of pages	13
Journal	International Archives of Allergy and Applied Immunology
Volume	46
Issue number	4
DOIs	https://doi.org/10.1159/000231156
State	Published - 1974

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Simple methods of rabbit hemolytic complement component assays

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