Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome

Keyphrases

• TALEN 100%
• Specific mutation 100%
• Zebrafish Genome 100%
• Locus-specific 100%
• Zebrafish 33%
• Site-directed mutation 33%
• Gene Function 22%
• Sequence Specificity 22%
• DNA Sequencing 11%
• Vertebrates 11%
• Adulthood 11%
• DNA Double-strand Breaks 11%
• Cloning Strategy 11%
• DNA Lesions 11%
• Multi-target 11%
• Targeted Sequencing 11%
• Sequence Modification 11%
• Sequence Polymorphism 11%
• DNA Recognition 11%
• Insertion mutation 11%
• Deletion mutation 11%
• Germline mutation 11%
• Off-target Effects 11%
• Host Genome 11%
• Custom Design 11%
• DNA-binding Specificity 11%
• Web-based Software 11%
• High-resolution Melting Analysis 11%
• Simple Cloning 11%
• Synthetic nucleases 11%

Biochemistry, Genetics and Molecular Biology

• Zebra Fish 100%
• Induced Mutation 100%
• Gene Function 40%
• RNA 20%
• Indel 20%
• Gene Mutation 20%
• DNA Sequence 20%
• DNA Damage 20%
• DNA Strand 20%
• DNA Binding 20%
• Germline Mutation 20%
• Nuclease 20%