Silver nitrate cauterization: Characterization of a new model of corneal inflammation and hyperalgesia in rat

H. N. Wenk, Christopher N Honda

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Chemical cauterization of the central cornea with silver nitrate was assessed as a superficial injury model of tissue sensitization accompanying acute inflammation. Adult male Sprague-Dawley rats were anesthetized with halothane gas, and the centers of their right corneas treated with a silver nitrate applicator stick (75% silver nitrate, 25% potassium nitrate) to produce a discrete lesion 1 mm in diameter. Edema of the corneal stroma and elevated immune cell counts became significant 4 h after cauterization, and were still evident after 48 h. Behavioral sensitization to chemical stimuli was determined by counting the number of blinks following application of 1 μM capsaicin directly to the corneal surface. A significant increase in stimulus-induced blinking was evident 2 h after cauterization. Chemical sensitization peaked at 6 h, and was no longer significant at 12 h. We conclude that silver nitrate cauterization produces acute corneal inflammation and hyperalgesia, and may prove a useful model for the study of primary afferent nociceptors.

Original languageEnglish (US)
Pages (from-to)393-401
Number of pages9
JournalPain
Volume105
Issue number3
DOIs
StatePublished - Oct 2003

Keywords

  • Cornea
  • Hyperalgesia
  • Inflammation

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