Silica nanoparticles induce conformational changes of tau protein and oxidative stress and apoptosis in neuroblastoma cell line

Zahra Roshanfekrnahzomi; Paria Badpa; Behnaz Esfandiari; Saba Taheri; Mina Nouri; Keivan Akhtari; Koorosh Shahpasand; Mojtaba Falahati

doi:10.1016/j.ijbiomac.2018.09.118

Silica nanoparticles induce conformational changes of tau protein and oxidative stress and apoptosis in neuroblastoma cell line

Zahra Roshanfekrnahzomi
, Paria Badpa
, Behnaz Esfandiari
, Saba Taheri
, Mina Nouri
, Keivan Akhtari
, Koorosh Shahpasand
, Mojtaba Falahati

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The adverse effects of SiO₂ NPs on the biological systems like nervous system have not been well explored. This study aimed to evaluate the toxicity of SiO₂ NPs on the nervous system in vitro. Therefore, human tau protein and neuroblastoma cell line (SH-SY5Y) were used as targets. In this study we examined the side effects of SiO₂ NPs on tau protein structure using several techniques including CD, ANS fluorescence, UV–vis (360 nm), Congo red absorbance, TEM, and molecular dynamic. Also, the cytotoxicity effects of SiO₂ NPs against SH-SY5Y cell line were evaluated using MTT, ROS and apoptotic assays. Spectroscopic and molecular dynamic investigations indicated that natively unfolded structure of tau in the presence of SiO₂ NPs experienced a partially folded and amorphous aggregated structure. Cellular assay demonstrated that SiO₂ NPs exerted cytotoxic effect on SH-SY5Y cells through ROS accumulation and induction of apoptosis. Overall, these findings proved that SiO₂ NPs could induce adverse effects on tau structure and SH-SY5Y cell integrity. Moreover, further studies are required to elucidate the molecular mechanism of SiO₂ NPs-induced side effects in vivo.

Original language	English (US)
Pages (from-to)	1312-1320
Number of pages	9
Journal	International Journal of Biological Macromolecules
Volume	124
DOIs	https://doi.org/10.1016/j.ijbiomac.2018.09.118
State	Published - Mar 1 2019
Externally published	Yes

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Publisher Copyright:
© 2018 Elsevier B.V.

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Flow cytometry
Molecular dynamics
Neuroblastoma cell line
Silica nanoparticle
Spectroscopy
Tau

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10.1016/j.ijbiomac.2018.09.118

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title = "Silica nanoparticles induce conformational changes of tau protein and oxidative stress and apoptosis in neuroblastoma cell line",

abstract = "The adverse effects of SiO2 NPs on the biological systems like nervous system have not been well explored. This study aimed to evaluate the toxicity of SiO2 NPs on the nervous system in vitro. Therefore, human tau protein and neuroblastoma cell line (SH-SY5Y) were used as targets. In this study we examined the side effects of SiO2 NPs on tau protein structure using several techniques including CD, ANS fluorescence, UV–vis (360 nm), Congo red absorbance, TEM, and molecular dynamic. Also, the cytotoxicity effects of SiO2 NPs against SH-SY5Y cell line were evaluated using MTT, ROS and apoptotic assays. Spectroscopic and molecular dynamic investigations indicated that natively unfolded structure of tau in the presence of SiO2 NPs experienced a partially folded and amorphous aggregated structure. Cellular assay demonstrated that SiO2 NPs exerted cytotoxic effect on SH-SY5Y cells through ROS accumulation and induction of apoptosis. Overall, these findings proved that SiO2 NPs could induce adverse effects on tau structure and SH-SY5Y cell integrity. Moreover, further studies are required to elucidate the molecular mechanism of SiO2 NPs-induced side effects in vivo.",

keywords = "Flow cytometry, Molecular dynamics, Neuroblastoma cell line, Silica nanoparticle, Spectroscopy, Tau",

author = "Zahra Roshanfekrnahzomi and Paria Badpa and Behnaz Esfandiari and Saba Taheri and Mina Nouri and Keivan Akhtari and Koorosh Shahpasand and Mojtaba Falahati",

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year = "2019",

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doi = "10.1016/j.ijbiomac.2018.09.118",

language = "English (US)",

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T1 - Silica nanoparticles induce conformational changes of tau protein and oxidative stress and apoptosis in neuroblastoma cell line

AU - Roshanfekrnahzomi, Zahra

AU - Badpa, Paria

AU - Esfandiari, Behnaz

AU - Taheri, Saba

AU - Nouri, Mina

AU - Akhtari, Keivan

AU - Shahpasand, Koorosh

AU - Falahati, Mojtaba

PY - 2019/3/1

Y1 - 2019/3/1

N2 - The adverse effects of SiO2 NPs on the biological systems like nervous system have not been well explored. This study aimed to evaluate the toxicity of SiO2 NPs on the nervous system in vitro. Therefore, human tau protein and neuroblastoma cell line (SH-SY5Y) were used as targets. In this study we examined the side effects of SiO2 NPs on tau protein structure using several techniques including CD, ANS fluorescence, UV–vis (360 nm), Congo red absorbance, TEM, and molecular dynamic. Also, the cytotoxicity effects of SiO2 NPs against SH-SY5Y cell line were evaluated using MTT, ROS and apoptotic assays. Spectroscopic and molecular dynamic investigations indicated that natively unfolded structure of tau in the presence of SiO2 NPs experienced a partially folded and amorphous aggregated structure. Cellular assay demonstrated that SiO2 NPs exerted cytotoxic effect on SH-SY5Y cells through ROS accumulation and induction of apoptosis. Overall, these findings proved that SiO2 NPs could induce adverse effects on tau structure and SH-SY5Y cell integrity. Moreover, further studies are required to elucidate the molecular mechanism of SiO2 NPs-induced side effects in vivo.

AB - The adverse effects of SiO2 NPs on the biological systems like nervous system have not been well explored. This study aimed to evaluate the toxicity of SiO2 NPs on the nervous system in vitro. Therefore, human tau protein and neuroblastoma cell line (SH-SY5Y) were used as targets. In this study we examined the side effects of SiO2 NPs on tau protein structure using several techniques including CD, ANS fluorescence, UV–vis (360 nm), Congo red absorbance, TEM, and molecular dynamic. Also, the cytotoxicity effects of SiO2 NPs against SH-SY5Y cell line were evaluated using MTT, ROS and apoptotic assays. Spectroscopic and molecular dynamic investigations indicated that natively unfolded structure of tau in the presence of SiO2 NPs experienced a partially folded and amorphous aggregated structure. Cellular assay demonstrated that SiO2 NPs exerted cytotoxic effect on SH-SY5Y cells through ROS accumulation and induction of apoptosis. Overall, these findings proved that SiO2 NPs could induce adverse effects on tau structure and SH-SY5Y cell integrity. Moreover, further studies are required to elucidate the molecular mechanism of SiO2 NPs-induced side effects in vivo.

KW - Flow cytometry

KW - Molecular dynamics

KW - Neuroblastoma cell line

KW - Silica nanoparticle

KW - Spectroscopy

KW - Tau

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ER -

Silica nanoparticles induce conformational changes of tau protein and oxidative stress and apoptosis in neuroblastoma cell line

Abstract

Bibliographical note

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