SILAC-based quantification of Sirt1-responsive Lysine acetylome

Yue Chen, Gozde Colak, Yingming Zhao

Research output: Chapter in Book/Report/Conference proceedingChapter

10 Scopus citations


Stable Isotope Labeling by Amino acids in Cell culture (SILAC) is one of the in vivo metabolic labeling methods widely used for dynamic analysis of protein modifications. Here, we describe a general approach to applying SILAC, in combination with affinity enrichment of acetyllysine peptides and mass spectrometry, to study the dynamic changes of the Lysine acetylome in response to Sirt1. The method should be applicable to quantify changes to other post translational modifications in diverse cellular systems.

Original languageEnglish (US)
Title of host publicationSirtuins
Subtitle of host publicationMethods and Protocols
EditorsMatthew Hirschey
Number of pages16
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Immunoaffinity purification
  • Lysine acetylation
  • Nano HPLC mass spectrometry
  • Quantification
  • Sirt1


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