SILAC-based quantification of Sirt1-responsive Lysine acetylome

Yue Chen, Gozde Colak, Yingming Zhao

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

Stable Isotope Labeling by Amino acids in Cell culture (SILAC) is one of the in vivo metabolic labeling methods widely used for dynamic analysis of protein modifications. Here, we describe a general approach to applying SILAC, in combination with affinity enrichment of acetyllysine peptides and mass spectrometry, to study the dynamic changes of the Lysine acetylome in response to Sirt1. The method should be applicable to quantify changes to other post translational modifications in diverse cellular systems.

Original languageEnglish (US)
Title of host publicationSirtuins
Subtitle of host publicationMethods and Protocols
EditorsMatthew Hirschey
Pages105-120
Number of pages16
DOIs
StatePublished - Sep 20 2013

Publication series

NameMethods in Molecular Biology
Volume1077
ISSN (Print)1064-3745

Keywords

  • Immunoaffinity purification
  • Lysine acetylation
  • Nano HPLC mass spectrometry
  • Quantification
  • SILAC
  • Sirt1

Fingerprint Dive into the research topics of 'SILAC-based quantification of Sirt1-responsive Lysine acetylome'. Together they form a unique fingerprint.

Cite this