Shrink polymer based electrochemical sensor for point-of-care detection of prostate-specific antigen

Wenzheng He, Liying Liu, Zeyuan Cao, Yang Lin, Yuxin Tian, Qifu Zhang, Changdong Zhou, Xiongying Ye, Tianhong Cui

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

There is a growing but unmet need for point-of-care detection of prostate-specific antigen (PSA) in body fluid which may facilitate early diagnosis and therapy of prostate cancer in a cost-effective and user-friendly way. Low sensitivity and narrow detection range limits applications of point-of-care testing in practice. Here, an immunosensor is first presented based on shrink polymer and integrated into a miniaturized electrochemical platform for detecting PSA in clinical samples. The sensing electrode was prepared by sputtering a gold film on shrink polymer, followed by heating to shrink the electrode to a small size with wrinkles from nano-scale to micro-scale. These wrinkles can be directly regulated by the thickness of the gold film with high specific areas for enhancement of antigen-antibody binding (3.9 times). A distinct difference between electrochemical active surface area (EASA) and response to PSA of shrink electrodes was observed and discussed. The electrode was treated with air plasma and modified with self-assembled graphene to further enhance the sensor's sensitivity (10.4 times). The shrink sensor with gold 200 nm thick integrated into the portable system was validated by a label-free immunoassay for detection of PSA in 20 μL serum within 35 mins. It exhibited a limit of detection of 0.38 fg/mL, the lowest among label-free PSA sensors, and a wide linear response from 10 fg/mL to 1000 ng/mL. Moreover, the sensor demonstrated reliable assay results in clinical serums, comparable to the commercial chemiluminescence instrument, confirming its feasibility for clinical diagnosis.

Original languageEnglish (US)
Article number115193
JournalBiosensors and Bioelectronics
Volume228
DOIs
StatePublished - May 15 2023
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2023 Elsevier B.V.

Keywords

  • Biomarkers
  • Electrochemical immunosensor
  • Label-free immunoassay
  • Micro/nano-structures
  • Point-of-care diagnosis
  • Shrink polymer

PubMed: MeSH publication types

  • Journal Article

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