Shorter Duration Time Trial Performance and Recovery Is Not Improved by Inclusion of Protein in a Multiple Carbohydrate Supplement

Anthony S. Wolfe, Sallee A. Brandt, Isaac A. Krause, Rachel W. Mavison, Josh A. Aponte, Lisa M. Ferguson-Stegall

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Shorter duration time trial performance and recovery is not improved by inclusion of protein in a multiple carbohydrate supplement. J Strength Cond Res 31(9): 2509-2518, 2017 - Ingesting multiple carbohydrate (CHO) types during exercise can improve endurance performance compared with single CHO only. Adding protein to a multiple CHO beverage has been shown to increase cycling time to exhaustion (TTE) compared with a single CHO beverage. However, it is unclear if improvements were due to multiple CHO or protein, and TTE protocols are not representative of typical race events. This study investigated whether adding protein to a multiple CHO beverage improved performance and recovery in 2 same-day cycling time trials (TTs) compared with isocaloric multiple CHO only. Ten cyclists (37.4 ± 8.9 years; Vo 2 max 54.6 ± 6.5 ml·kg -1 ·min -1) performed a familiarization and 2 randomized, crossover, double-blinded experimental trials consisting of pretrial leg strength testing, 40-km TT, 30-min recovery, 10-km TT, and posttrial leg strength testing. Seven 275 ml doses of multiple CHO (MCO) or multiple CHO+protein (MCP) were ingested during the protocol. Blood glucose, lactate, heart rate (HR), and rating of perceived exertion (RPE) were also measured. Continuous variables were analyzed with paired t-tests, and repeated measures with repeated-measures analysis of variance. No differences existed between MCO and MCP in 40-km TT time (81.6 ± 2.8 vs. 81.9 ± 2.9 minutes, respectively, p = 0.94), or in 10-km time (24.0 ± 0.9 vs. 23.9 ± 1.0 minutes, p = 0.97). Blood glucose was higher before 10-km TT in MCO compared with MCP (3.78 ± 0.20 vs. 3.31 ± 0.19 mmol·L -1, p = 0.002). No treatment differences were found for lactate, HR, RPE, or strength recovery. When using a protocol and performance measures that replicate realistic, shorter duration events, adding protein to a multiple CHO beverage does not improve performance compared with multiple CHO only.

Original languageEnglish (US)
Pages (from-to)2509-2518
Number of pages10
JournalJournal of strength and conditioning research
Issue number9
StatePublished - Sep 1 2017
Externally publishedYes

Bibliographical note

Funding Information:
The authors thank our subjects for their time, enthusiasm, effort, and dedication. They also thank Ambassador Ridg-way for her support of collaborative research. Funding was also provided by a Howard Hughes Medical Institute grant to the Biology Department. The authors declare no conflicts of interest. The results of this study do not constitute endorsement by the authors or the NSCA.

Publisher Copyright:
© 2016 National Strength and Conditioning Association.


  • cycling
  • second bout
  • supplementation


Dive into the research topics of 'Shorter Duration Time Trial Performance and Recovery Is Not Improved by Inclusion of Protein in a Multiple Carbohydrate Supplement'. Together they form a unique fingerprint.

Cite this